Welcome to the open zeta version of XDS-J! XDS-J is a graphical user interface for XDS Recommended resolution:1280×960, low resolution may cause incompletely display(Notice there are scrollbars on the top side and the right side) Steps : 1.Open a terminal, change directory, and input command: XDS-J. 2.Click 'browse' on the right side of 'image', and choose one image from a certain dataset.(not necessary to be the first image) 3.Choose detecor type and relative parameters. Parameters for beamlines 17U, 18U, 19U at Shanghai Synchrotron Radiation Facility have been stored, click the button to automatically enter. 4.Click 'run xds for ? rounds'. If XDS runs successfully, 'Finished' in red will appear on the top, results will appear in the text widget, and entries for SPACE_GROUP_NUMBER and UNIT_CELL_CONSTANTS will be automatically filled. 5.If you think the automatically determined space group is not correct, click 'show all possible space group', choose one from the popup menu in the top-level window and click 'confirm'. Modify the highest resolution according to the result in the text widget(If the checkbutton before 'I/SIGMA cutoff' is selected, Maxinum_Resolution will be modified based on the cutoff value after running XDS for one round). Click 'run xds for ? rounds' to run again. 6.Change 'Job' to 'DEFPIX INTEGRATE CORRECT' and run XDS for several times to optimize the results(you can change the number below 'run xds for ? rounds'). You can also modify other parameters such as DATA_RANGE(some data need to be excluded because of radiation damage) and WFAC1(to reject more points). Generally, the goal is to make I/SIGMA of the highest resolution close to 2, while CC(1/2) greater than 60, total COMPLETENESS greater than 80%, NUMBER OF REFLECTIONS OBSERVED is more than four times of the UNIQUE, and NUMBER OF REJECTED MISFITS less than 10% of NUMBER OF REFLECTIONS IN SELECTED SUBSET OF IMAGES.(these figures are only for reference) 7.Click 'xscale&xdsconv'. In the top-level window, click 'xscale', and then click 'xdsconv'. The finally mtz file that can be used for CCP4 will be generated in the current directory. Notice : When running XDS, the entire graphical interface will be temporarily locked. Do not carry out any operation. Running the program will generate a lot of files, so it is better to run XDS-J in a separated directory. If the results show that I/SIGMA of every resolution range is very low, it is possible that the crystal position (ORGX, ORGY) deviates greatly. Possible causes of failure: the quality of your data is not good (you can view the image through 'view' button to check), wrong rotation axis, the highest resolution is too small(such as 0), wrong space group(Different DATA_RANGE may lead to different automatic determination of the space group.) Ways to improve I/SIGMA: use smaller WAC1(such as 0.85), change DATA_RANGE, run XDS repeatedly.