Useful Websites
Useful Websites
Release time:2019-11-16 Browse:52

一、蛋白质分析(protein prediction)


1. 信号肽预测(Signal peptide prediction)

http://www.cbs.dtu.dk/services/SignalP/


2. 蛋白性质预测(Protein property prediction)

http://web.expasy.org/protparam/ 


3. 二级结构预测(Secondary structure prediction)

http://bioinf.cs.ucl.ac.uk/psipred/      

                             

http://www.compbio.dundee.ac.uk/~www-jpred/index.html


4. 跨膜区预测TMpred(Transmembrane domain prediction TMpred)

http://www.ch.embnet.org/software/TMPRED_form.html


5. 三维结构比对Three-dimensional structure comparison

http://ekhidna.biocenter.helsinki.fi/dali_server/start


6.结构域预测Domain prediction 

http://smart.embl-heidelberg.de/


7.天然无结构预测Natural unstructured prediction

http://www.disprot.org/predictors.php


8.信号肽、跨膜区预测Signal peptide, transmembrane region prediction

http://www.cbs.dtu.dk/services/TMHMM-2.0/


9.与同源蛋白的结构比较,做一个MultAlin, 去除N端和C端不保守的柔性区域 (Compared with the structure of homologous proteins, make a MultAlin, remove the flexible regions that are not conserved at the N-terminus and C-terminus.)              

http://bioinfo.genopoletoulouse.prd.fr/multalin/multalin.html                                                  http://www.ncbi.nlm.nih.gov/PyMOL


10.稀有密码子预测(Rare codon prediction)

http://nihserver.mbi.ucla.edu/RACC/


11.更多信息学预测信息(More bioinformatic prediction information)

http://www.expasy.org/tools/

                         


二、分子克隆(molecular clone)


1.引物Tm值测定(Primer Tm value determination)

http://biotools.nubic.northwestern.edu/OligoCalc.html


2.密码子优化(Codon optimization)

https://sg.idtdna.com/CodonOpt


3.质粒分析(Plasmid analysis)

http://wishart.biology.ualberta.ca/PlasMapper/