一、蛋白质分析(protein prediction)
1. 信号肽预测(Signal peptide prediction)
http://www.cbs.dtu.dk/services/SignalP/
2. 蛋白性质预测(Protein property prediction)
http://web.expasy.org/protparam/
3. 二级结构预测(Secondary structure prediction)
http://bioinf.cs.ucl.ac.uk/psipred/
http://www.compbio.dundee.ac.uk/~www-jpred/index.html
4. 跨膜区预测TMpred(Transmembrane domain prediction TMpred)
http://www.ch.embnet.org/software/TMPRED_form.html
5. 三维结构比对(Three-dimensional structure comparison)
http://ekhidna.biocenter.helsinki.fi/dali_server/start
6.结构域预测(Domain prediction)
http://smart.embl-heidelberg.de/
7.天然无结构预测(Natural unstructured prediction)
http://www.disprot.org/predictors.php
8.信号肽、跨膜区预测(Signal peptide, transmembrane region prediction)
http://www.cbs.dtu.dk/services/TMHMM-2.0/
9.与同源蛋白的结构比较,做一个MultAlin, 去除N端和C端不保守的柔性区域 (Compared with the structure of homologous proteins, make a MultAlin, remove the flexible regions that are not conserved at the N-terminus and C-terminus.)
http://bioinfo.genopoletoulouse.prd.fr/multalin/multalin.html http://www.ncbi.nlm.nih.gov/PyMOL
10.稀有密码子预测(Rare codon prediction)
http://nihserver.mbi.ucla.edu/RACC/
11.更多信息学预测信息(More bioinformatic prediction information)
二、分子克隆(molecular clone)
1.引物Tm值测定(Primer Tm value determination)
http://biotools.nubic.northwestern.edu/OligoCalc.html
2.密码子优化(Codon optimization)
https://sg.idtdna.com/CodonOpt
3.质粒分析(Plasmid analysis)
http://wishart.biology.ualberta.ca/PlasMapper/