2018 TJ-Lab Training Completed
Our annual TJ-Lab research training started on August 6th and ended on August 9th. This year, over 20 guests from our collaborators and friends' lab joined our training program.
Edited by our Ph.D. candidate, Caiying Zhang, our 3rd edition of TJ-Lab SOP was used as our training reference. This year's training covers molecular biology, protein expression and purification, biochemical and biophysical assays, structural biology techniques, softare, scientific writing and ended with an evaluation exam.
Overall, this year's training was very successful. Many participants expressed their appreciation for the hard-working of our organizers, SOP authors and presenters.
2018 TJ-Lab Summer Training Schedule
Time | Topics | Presenter | time | |
8.6 | ||||
a.m.
| 9:00-9:30 | How to do research | 金老师 | 30 min |
9:30-9:50 | Vectors and cells | 赵丹 | 20 min | |
9:50-10:10 | Group photo | 20 min | ||
10:10-10:50 | Molecular cloning | 范晓娇 | 40 min | |
10:50-11:10 | Gibson assembly | 范晓娇 | 20 min | |
11:10-11:40 | Site-directed mutagenesis | 范晓娇 | 30 min | |
p.m.
| 14:30-15:00 | Columns and maintenance | 周家慧 | 30 min |
15:00-15:30 | AKTA FPLC machine | 周家慧 | 30 min | |
15:30-15:50 | AKTA FPLC demo | 房康 | 20 min | |
16:00-16:40 | Protein purification | 曾威红 | 40 min | |
16:40-17:00 | Refolding | 张宏亮 | 20 min | |
17:00-17:20 | Purification of antibody | 张宏亮 | 20 min | |
17:20-17:40 | SeMet labeling | Abdus | 20 min | |
8.7 | ||||
a.m.
| 9:00-9:40 | Insect expression system | Ayesha | 40 min |
9:40-10:00 | Tissue culture | 刘木子樱 | 20 min | |
10:10-10:40 | Y2H screening | 马欢 | 30 min | |
10:40-11:10 | Phage display | 黄晓雪 | 30 min | |
11:10-11:40 | SELEX | Abdus | 30 min | |
p.m.
| 14:30-14:45 | Y2H(vivo) | 李越龙 | 15 min |
14:45-15:00 | BiFc(vivo) | 李越龙 | 15 min | |
15:00-15:15 | ELISA | 黄晓雪 | 15 min | |
15:15-15:30 | Crosslink | 李文倩 | 15 min | |
15:30-15:55 | Co-IP and Western Blotting(+video) | 李文倩 | 25 min | |
15:55-16:10 | Gel filtration and native gel | 李文倩 | 15 min | |
16:20-16:35 | EMSA | 张彩英 | 15 min | |
16:35-16:50 | FP | 张彩英 | 15 min | |
16:50-17:05 | SPR | 刘木子樱 | 15 min | |
17:05-17:20 | DSF | 尹雪莹 | 15 min | |
17:20-17:40 | ITC | 陈凤 | 20 min | |
8.8 | ||||
a.m.
| 9:00-9:20 | Prism | 徐志豪 | 20 min |
9:20-9:40 | Lasergene | 徐志豪 | 20 min | |
9:40-10:00 | Scientific writing and Endnote | 金老师 | 20 min | |
10:10-10:25 | CD Spectrum | 李越龙 | 15 min | |
10:25-10:45 | SAXS | 尹雪莹 | 20 min | |
10:45-11:10 | CryoEM | 陈凤 | 25 min | |
11:10-11:40 | X-ray crystallography theory | 金老师 | 30 min | |
p.m.
| 14:30-14:50 | Sample preparation | 金老师 | 20 min |
14:50-15:20 | Crystallization methods | 金老师 | 30 min | |
15:20-15:30 | Crystallization kits | 金老师 | 10 min | |
15:30-16:00 | Optimization and salt crystals | 金老师 | 30 min | |
16:10-16:20 | Cryocrystallography | 金老师 | 10 min | |
16:20-16:40 | X-ray diffraction data collection | 金老师 | 20 min | |
16:40-17:40 | Data processing and evaluation(HKL2000,XDS) | 金老师 | 1h | |
8.9 | ||||
a.m.
| 9:00-10:00 | Phasing methods (SAD/MAD/MR, CCP4,phenix) | 朱老师 | 1h |
10:10-10:40 | Manual model building with COOT | 金老师 | 30 min | |
10:40-11:10 | Structural refinement | 金老师 | 30 min | |
11:10-11:40 | Structure valildation and deposition | 金老师 | 30 min | |
p.m.
| 14:30-15:00 | Molecular graphics with Pymol | 金老师 | 30 min |
15:10-17:10 | Examination | 2h | ||
17:20-17:30 | Summary | 金老师 | 10 min | |
