NEWS
2018 TJ-Lab Training Completed
Release time:2018-10-17 Browse:29


Our annual TJ-Lab research training started on August 6th and ended on August 9th. This year, over 20 guests from our collaborators and friends' lab joined our training program.

       Edited by our Ph.D. candidate, Caiying Zhang, our 3rd edition of TJ-Lab SOP was used as our training reference. This year's training covers molecular biology, protein expression and purification, biochemical and biophysical assays, structural biology techniques, softare, scientific writing and ended with an evaluation exam.

       Overall, this year's training was very successful. Many participants expressed their appreciation for the hard-working of our organizers, SOP authors and presenters.


2018 TJ-Lab Summer Training Schedule 



Time

Topics

Presenter

time

8.6





a.m.

  

  

  

  

  


9:00-9:30

How   to do research

金老师

30 min

9:30-9:50

Vectors   and cells

赵丹

20 min

9:50-10:10

Group photo


20 min





10:10-10:50

Molecular   cloning

范晓娇

40 min

10:50-11:10

Gibson   assembly

范晓娇

20 min

11:10-11:40

Site-directed   mutagenesis

范晓娇

30 min

p.m.

  

  

  

  

  

  


14:30-15:00

Columns   and maintenance

周家慧

30 min

15:00-15:30

AKTA FPLC machine

周家慧

30 min

15:30-15:50

AKTA FPLC demo

房康

20 min





16:00-16:40

Protein purification

曾威红

40 min

16:40-17:00

Refolding

张宏亮

20 min

17:00-17:20

Purification of antibody

张宏亮

20 min

17:20-17:40

SeMet labeling

Abdus

20 min

8.7





a.m.

  

  

  

  


9:00-9:40

Insect   expression system

Ayesha

40 min

9:40-10:00

Tissue   culture

刘木子樱

20 min





10:10-10:40

Y2H screening

马欢

30 min

10:40-11:10

Phage display

黄晓雪

30 min

11:10-11:40

SELEX

Abdus

30 min

p.m.

  

  

  

  

  

  

  

  

  

  

  


14:30-14:45

Y2H(vivo)

李越龙

15 min

14:45-15:00

BiFc(vivo)

李越龙

15 min

15:00-15:15

ELISA

黄晓雪

15 min

15:15-15:30

Crosslink

李文倩

15 min

15:30-15:55

Co-IP and Western Blotting(+video)

李文倩

25 min

15:55-16:10

Gel filtration and native gel

李文倩

15 min





16:20-16:35

EMSA

张彩英

15 min

16:35-16:50

FP

张彩英

15 min

16:50-17:05

SPR

刘木子樱

15 min

17:05-17:20