SOP
6. 附录
Release time:2019-11-16 Browse:29

6 附录... 289

6.1.1常用抗生素配方... 289

6.1.2常用母液配方... 290

6.1.3缓冲液(pH 6-10)pKa及缓冲范围... 293

6.1.4磷酸缓冲液配方... 294

6.2.1常用网站... 296

6.2.2通用测序引物... 297

6.2.3密码子及其偏好性表格... 298

6.2.4空间群表格... 301

6.2.5线站新衍射仪BL17U1. 302

6.2.6实验室载体... 302

6.2.7实验室细胞株及菌株... 307

6.2.8结晶试剂盒.... 310

抗生素

母液浓度(mg/mL

工作浓度μg/mLfor

严紧型质粒

工作浓度(μg/mLfor

松驰型质粒

处理方式

Ampicillin,氨苄青霉素(Amp)

100(溶于水)

20

60

过滤除菌

Kanamycin,卡那霉素(Kan)

50 (溶于水)

10

50

过滤除菌/高压

Chloramphenicol,氯霉素(Chl/Cm/CAP)

34 (溶于乙醇)

 

25

170

无需灭菌

Carbenicillin,羧苄青霉素(Cab)

50 (溶于水)

20

60

过滤除菌

Streptomycin,链霉素(Sm)

10 (溶于水)

10

50

过滤除菌

Tetracyyline,四环素(Tet)

5 (溶于甲醇)

10

50

无需灭菌





















1.   保存条件:建议 -20 °C保存。

2.    mycin,霉素,在不严格的缩写中作为“XX霉素”的后缀缩写为m

3.    氯霉素(Chlor Am Phenicol),氯氨苯醇,标准缩写CAP,有时也写做Cm

4.    过滤灭菌:用0.22 μm一次性滤膜过滤除菌,于超净台内。

5.   母液配制方法举例,如100 mg/mL氨苄青霉素:溶解1 g氨苄青霉素钠盐于足量的水中,最后定容至10 mL。分装成小份于-20 °C贮存。常以25 μg/mL50 μg/mL的终浓度添加于生长培养基

1.   50× TAE1 L):

Tris                                                          242 g

Glacial Acetic Acid (冰醋酸)                 57.1 mL

0.5 M EDTA                                           100 mL

2.    10× TBE1 L):

Tris                                                           108.8 g       

Boric Acid                                                55.0 g             

0.5 M EDTA                                            40 mL                

3.    6×DNA Loading Buffer100 mL):

1 M Tris-HCl pH 7.4                                  10 mL

100% Glycerol                                           60 mL

0.5 M EDTA                                              12 mL

Bromophenol Blue                                     0.01 g

4.   0.5 M EDTA pH 8.0500 mL):

EDTA Disodium Salt Dihydrate               93.05 g  

Dissolve with about 700 mL ddH2O, stir, then adjust pH to 8.0 (1 M NaOH)

Adjust the volume to 1 L. Filtered with 0.22 μm membrane or autoclave.

**EDTA does not dissolve until nearly pH 8.0

5.   X-Gal 40 mg/mL, 1000×5 mL

X-Gal                                                          0.200 g

Dimethyl Foramide                                      5 mL

use glass pipette!

Make ~500 μL aliquots.  Store in brown glass vials at -20 °C.

6.   SOC medium250 mL

Tryptone Peptone                                          5 g

Yeast extract                                                  1.25 g

NaCl                                                              0.15 g

KCl10 mM                                            0.05 g

MgCl210 mM                                       0.51 g

50% glucose                                                   4 mL

add sterile and MgCl2 and glucose after autoclaving

7.    Solutions for Plasmid Preparations

1)    Qiagen Buffer P150 mM Tris-HCl pH 8.010 mM EDTA100 μg/mL RNaseA

&for resuspension of cells in DNA plasmid preparation. 和生工P1通用。

1 M Tris pH 8.0                                               5 mL       

0.5 M EDTA pH 8.0                                        2 mL

add ddH2O to 100 mL, Autoclave. Rnase A (add after autoclaving)  10.0 mg. Store at 4 °C

2)    Qiagen Buffer P2200 mM NaOH1% SDS

&for lysis of cells in DNA plasmid preparation. 和生工P2通用。

NaOH                                                               0.8 g

SDS                                                                  1.0 g

add ddH2O to 100 mL

3)     Qiagen Buffer P33.0 M potassium acetate pH 5.5,

&not for spin columns, but for Qiatips, midi, maxi, giga kits

Potassium Acetate                                  25.03 g

Acetic Acid, Galacial                ~11.5 mL

Adjust pH to 5.5, add ddH2O to 100 mL

4)     Qiagen Buffer N34.2 M Gu-HCl0.9 M potassium acetate pH 4.8

&as nutrealization buffer to precipitate proteins and genomic materials,和生工小抽试剂盒P3通用。

5)     Qiagen Buffer PB5 M Gu-HCl, 30% isopropanol)=PBII for pH indicator

6)     Qiagen Buffer QG5.5 M guanidine thiocyanate GuSCN),20 mM Tris HCl pH 6.6

7)     Qiagen Buffer PE10 mM Tris-HCl pH 7.580% ethanol

&for spin column salt wash in miniprep blueor pink columns. 用于质粒小抽和核酸提取离心柱清洗除盐。

8)     Qiagen Buffer QX17 M NaPO410 mM NaAc pH 5.3

&for solution and binding of agarose gels,用在用琼脂糖珠子DNA纯化试剂盒。

9)      Qiagen Buffer QXB5 M GuHCl

&for binding of large >3000 bp fragments to columns

10)     Qiagen Buffer QBT750 mM NaCl50 mM MOPS pH 7.015% isopropanol0.15% triton X-100

&Equilibration buffer

NaCl                                                                 4.38 g

Isopropanol                                                      15 mL

MOPS                                                               1.05 g

Triton X-100                                                     150 mL

Adjust pH to 7.0use 1N, add ddH2O to 100 mL

11)     Qiagen Buffer QC1.0 M NaCl50 mM MOPS pH 7.015% isopropanol

&Wash buffer

NaCl                                                                 5.84 g

MOPS                                                              1.05 g

Isopropanol                                                      15 mL

Adjust pH to 7.0, add ddH2O to 100 mL ,filter sterilize.

12)     Qiagen Buffer QF1.25 M NaCl 50 mM Tris-HCl pH 8.515% isopropanol

&Elution buffer

NaCl                                                            7.31 g

Tris 8.5 1 M                                                 5 mL

Isopropanol                                                 15 mL

Adjust pH to 8.5, add ddH2O to 100 mL

8.     Buffers for Protein Electrophoresis

1)    6×SDS Sample Buffer

1 M Tris-HCl pH 6.8                   25 mL

10% SDS                                     20 mL

50% Glycerol                              50 mL

1 M DTT                                      2 mL

Bromophenol Blue                     0.01 g

Add ddH2O to 100 mL

2)   Coommassie Blue Staining Solution500 mL):

Coommassie Brilliant Blue R250                     0.5 g

Ethanol                                                           200 mL

Acetic acid:                                                      50 mL

ddH2O                                                              to 500 mL~250 mL

Dissolve Coommassie in Ethanol before adding water and acetic acid will require stirring 1~2 hours

3)   Coommassie Blue Destaining Solution 500 mL):

Ethanol                                     200 mL                           

Acetic Acid                               50 mL

ddH2O                                      250 mL

4)    20×MES-SDS running buffer 1 L):MES2-(N-吗啉)乙磺酸一水合物

&For high-resolution separation of small size proteins.

MES                                                   195.2 g  

Tris                                                     121.2 g

SDS                                                      20 g

EDTA                                                     6 g

9.    Molecular Biology Buffers:

1)    A11×NEBuffer 1yellow):10 mM Bis Tris Propane-HCl10 mM MgCl21 mM DTT pH 7.0 @ 25 °CSupplied as a 10×concentrated stock.

2)    A21×NEBuffer 2blue):50 mM NaCl, 10 mM Tris-HCl10 mM MgCl21 mM DTT pH 7.9 @ 25 °CSupplied as a 10×concentrated stock.

3)   A21×NEBuffer 3red):100 mM NaCl, 50 mM Tris-HCl10 mM MgCl21 mM DTT pH 7.9 @ 25 °CSupplied as a 10×concentrated stock.

4)   A21×NEBuffer 4greencutsmart buffer):50 mM potassium acetate20 mM Tris acetate10 mM magnesium acetate1 mM DTT, pH 7.9 @ 25 °CSupplied as a 10×concentrated stock.

5)   2×T4 DNA quick ligase 快连接酶buffer60 mM Tris-HCl pH 7.820 mM MgCl22 mM DTT2 mM ATP and 10% PEG6000

6)   10×T4 DNA连接酶buffer500 mM Tris-HCl pH 7.6100 mM MgCl2100 mM DTT10 mM ATP

7)   10×pfu buffer200 mM Tris-HCl pH 8.6100 mM KCl160 mM (NH4)2SO410 mM MgCl21% Triton X-1001 mg/mL BSA

8)   5×HF buffer32 mM HEPES-KOH buffer pH 7.8100 mM KAcO, 4 mM Mg (AcO) 20.05% BSA0.5 mg/mL

9)   5×GC rich buffer32 mM HEPES-KOH buffer pH 7.8100 mM KAcO7.5 mM Mg(AcO)20.01% BSA0.1 mg/mL),1.0% DMSO

10)     2×Taq mix:

1 M Tris-HCl pH 8.6                               4 mL

1 M KCl                                                   20 mL

1 M MgCl2                                              600 μL

Sucrose                                                    68 g

dNTP 2 mM each                           40 mL

1% Cresol Red(甲酚红)                    2 mL

Taq polymerase 2U/μL                  20 mL

Add ddH2O to 200 mL

11)     10×Taq buffer:

200 mM Tris-HCl 8.4200 mM KC100 mM (NH4)2SO430 mM MgSO41 mg/mL BSA

6.1.3 缓冲液(pH 6-10)pKa及缓冲范围

缓冲液

pKa25°C

缓冲范围

分子量

中文全称

MES

6.1

5.5~6.7

195.2

2-吗啉乙磺酸

Bis-Tris

6.5

5.8~7.2

209.2

(2-羟乙基)亚胺基三(羟甲基)甲烷

ADA

6.6

6.0~7.2

190.2

N-(氨基甲酰基甲基)亚氨基二乙酸

ACES

6.8

6.1~7.5

182.2

N-氨基甲酰甲基乙磺酸

PIPES

6.8

6.1~7.5

302.4

哌嗪-NN'-(2-乙磺酸)

MOPSO

6.9

6.2~7.6

225.3

3-(N-吗啉基)-2-羟基丙磺酸

Bis-Tris Propane

6.8

6.3~9.5

282.3

[(羟甲基)氨基丙烷]/1,3-[(羟甲基)甲氨基]丙烷

BES

7.1

6.4~7.8

213.2

N-(2-羟乙基)-2-氨基乙璜酸

MOPS

7.2

6.5~7.9

209.3

3-(N-吗啡啉)乙磺酸

HEPES

7.5

6.8~8.2

238.3

N-2-羟乙基哌嗪-N'-2-乙磺酸

TES

7.4

6.8~8.2

229.2

N-3-(羟甲基)甲基-2-氨基乙磺酸

DIPSO

7.6

7.0~8.2

243.3

3-[N-N-(2-羟乙基)氨基]-2-羟基丙磺酸

TAPSO

7.6

7.0~8.2

259.3

N-3-(羟甲基)甲氨基-2-羟基丙烷磺酸

Tris

8.1

7.0~9.1

121.1

三羟甲基氨基甲烷

HEPPSO

7.8

7.1~8.5

268.3

4-(2-羟乙基)哌嗪-1-2-羟基丙磺酸

POPSO

7.8

7.2~8.5

362.4

哌嗪-1,4-二羟基丙磺酸

EPPS

8.0

7.3~8.7

252.3

4-羟乙基哌嗪丙磺酸

TEA

7.8

7.3~8.3

149.2

三乙醇胺

Tricine

8.1

7.4~8.8

179.2

N--(羟甲基)甲基氨基乙酸

Bicine

8.3

7.6~9.0

163.2

N,N-(2-羟乙基)甘氨酸

TAPS

8.4

7.7~9.1

243.3

三羟甲基甲胺基丙磺酸

AMPSO

9.0

8.3~9.7

227.3

3-[N-(11-二甲基-2-羟乙基)]氨基-2-羟丙烷磺酸

CHES

9.3

8.6~10.0

207.3

2-(环已胺)-1-乙磺酸

CAPSO

9.6

8.9~10.3

237.3

3-(环已胺)-2-羟基-1-丙磺酸

AMP

9.7

9.0~10.5

89.1

2-氨基-2-甲基-1-丙醇

CAPS

10.4

9.7~11.1

221.3

3-(环已胺)-1-丙磺酸

材料(MATERIALS

r  试剂(REAGENTS

NaH2PO4·2H2ONa2HPO4·12H2O

配制方法:

配制时,常先配制0.2 MNaH2PO40.2 MNa2HPO4,两者按一定比例混合即成0.2 M的磷酸盐缓冲液(PB),根据需要可配制不同浓度的PBPBS

1.  0.2 M NaH2PO4:称取NaH2PO4.2H2O 31.21 g(或 NaH2PO4·H2O 27.6 g)加重蒸水至1000 mL溶解。

2.  0.2 M Na2HPO4:称取Na2HPO4·12H2O 71.64 g(或 Na2HPO4·7H2O 53.6 g Na2HPO4·2H2O 35.61 g)加重蒸水至1000 mL溶解。

3.  0.2 M pH 7.4PB的配制:取19 mL 0.2 MNaH2PO481 mL 0.2 M Na2HPO4,充分混合即为0.2 MPBpH约为7.47.5)。若pH偏高或偏低,可通过改变二者的比例来加以调整,室温保存即可。

       25 °C0.2 M磷酸钠缓冲液的配制

pH

0.2 M Na2HPO4(mL)

0.2 M NaH2PO4(mL)

5.8

 8.0

 92.0

5.9

 10.0

 90.0

6.0

 12.3

 87.7

6.1

 15.0

 85.0

6.2

 18.5

 81.5

6.3

 22.5

 77.5

6.4

 26.5

 73.5

6.5

 31.5

 68.5

6.6

 37.5

 62.5

6.7

 43.5

 56.5

6.8

 49.0

 51.0

6.9

 55.0

 45.0

7.0

 61.0

 39.0

7.1

 67.0

 33.0

7.2

 72.0

 28.0

7.3

 77.0

 23.0

7.4

 81.0

 19.0

7.5

 84.0

 16.0

7.6

 87.0

 13.0

7.7

 89.5

 10.5

7.8

 91.5

 8.5

7.9

 93.5

 6.50

8.0

 94.7

 5.3

       25 °C0.1 M磷酸钾缓冲液的配制

pH

1 M K2HPO4(mL)

1 M KH2PO4(mL)

5.8

 8.5

 91.5

6.0

 13.2

 86.8

6.2

 19.2

 80.8

6.4

 27.8

 72.2

6.6

 38.1

 61.9

6.8

 49.7

 50.3

7.0

 61.5

 38.5

7.2

 71.7

 28.3

7.4

 80.2

 19.8

7.6

 86.6

 13.4

7.8

 90.8

 9.2

8.0

 94.0

 6.0

6.2 资源信息类

6.2.1 常用网站

常用网站根据功能分类如下:

1.         分子克隆(Molecular Cloning

1)         引物Tm值计算:http://biotools.nubic.northwestern.edu/OligoCalc.html

2)         密码子优化:https://sg.idtdna.com/CodonOpt

3)         质粒图谱分析:http://wishart.biology.ualberta.ca/PlasMapper/

4)         启动子信息:https://blog.addgene.org/plasmids-101-the-promoter-region

5)         addgene(一个质粒数据库):http://www.addgene.org/vector-database/

6)         NEB 内切酶buffer推荐:https://nebcloner.neb.com/#!/redigest

7)         核酸数据分析:http://biotools.nubic.northwestern.edu/OligoCalc.html

2.         蛋白表达与纯化(Protein Expression and Purification

1)         蛋白表达菌株信息:http://wolfson.huji.ac.il/expression/bac-strains-prot-exp.html

2)         蛋白纯化:http://wolfson.huji.ac.il/purification/Purification_Protocols.html

3.         蛋白序列分析(Protein Sequence Analysis

1)         信号肽预测:http://www.cbs.dtu.dk/services/SignalP/

2)         跨膜区预:TMpred http://www.ch.embnet.org/software/TMPRED_form.html

3)         结构域预测:http://smart.embl-heidelberg.de/

4)         蛋白性质预测:http://web.expasy.org/protparam/

              http://biotools.nubic.northwestern.edu/proteincalc.html

5)         蛋白质结构与功能预测:http://raptorx.uchicago.edu/

6)         二级结构预测:http://bioinf.cs.ucl.ac.uk/psipred/

7)         评估结构预测的准确性和可靠性:https://www.cameo3d.org/

8)         蛋白质结构和功能预测:https://open.predictprotein.org/

9)         三维结构比对http://ekhidna.biocenter.helsinki.fi/dali_server/start

10)     同源建模https://zhanglab.ccmb.med.umich.edu/I-TASSER/

11)     同源建模:https://swissmodel.expasy.org/

12)     预测蛋白相互作用:https://string-db.org/

13)     Pfam数据库是蛋白质家族的集合(根据结构域分类):http://pfam.xfam.org/

4.         结构分析Structure Analysis)

1)         RCSB PDB 数据库:http://www.rcsb.org/

2)         晶体界面分析 PISA serverhttp://www.ebi.ac.uk/pdbe/pisa/

3)         Deposition serverhttps://deposit-1.wwpdb.org/

4)         配体结构文件下载:http://www.rcsb.org/pdb/ligand/chemAdvSearch.do

5)         Bernhard Rupp的生物大分子晶体学网站http://www.ruppweb.org/Xray/101index.html

5.         其他

1)         ATCChttp://www.attc.org/

2)         化学分子性质:http://www.chemicalbook.com/ProductIndex_EN.aspx

3)         RNA修饰数据库:http://mods.rna.albany.edu/mods/modifications/

4)         Wikipediahttps://www.wikipedia.org/

5)         各类protocolshttp://openwetware.org/wiki/Protocols

6)         文献查询:https://sci-hub.tw/

6.2.2 通用测序引物

简介(INTRODUCTION

当进行菌落PCR时,如果目的片段没有成功转化至细菌中,针对插入片段的特异性引物可能会与细菌自身DNA进行非特异性结合,产生非特异性条带,从而对我们鉴定克隆是否成功构建产生干扰。因此我们常用载体上的引物进行鉴定。利用这种引物,我们既可以检测转入的质粒是否是空载体,也能估测连入载体的插入片段大小是否正确。

以下是我们实验室常用的几个通用引物,既可以做菌落PCR,也可以用于测序。

Primer name

Site

Sequence

Z5

T7 promoter

TACGACTCACTATAGGGGAATTGTGAGCG

Z6

T7 terminator

Z8

TEV and Sal I

gagaatctttatttccaaggttctgTCGAC

Z9

v52 Rev

GGCCAGTGAATTGTAATACGACTCACTATA

Z10

v55 Rev

GTTCTGATTTAATCTGTATCAGGCTGAAAA

Z11

pCold (v113, v114) Rev

aaatggcagggatcttagattctgtg

XY27

pcDNA3.1 For

taatacgactcactatagggagacccaagc

XY28

pcDNA3.1 Rev

tagaaggcacagtcgaggctgatca

y936

v108 Rev

ggctgattatgatcctctagtacttc

Z12

v104 For

aatttgtaatacgactcactatagggcga

Z13

v104 Rev

tttagaggccccaaggggttatgctagtt

Z14

v105 For

agatctttaatacgactcactatagggcga

Z15

v105 Rev

ttgagatggtgcacgatgcacagttgaa

Z16

V126, 127 For

agaacccactgcttactggcttatcgaaat

Z17

V126, 127 Rev

ctgatcagcgggtttaaactcaatggtgat

L22

pEGFP-N1 For

tctatataagcagagctggtttagtgaaccgtcag

L23

pEGFP-N1 Rev

tgcacgccgtaggtcagggtggtcac

L28

pCDH For

GAGCTCGTTTAGTGAACCGTCAGATCGCCT

L29

pCDH Rev

GTTCAATTGCCGACCCCTCCCCCCAACTTC

第一位碱基

第二位碱基

U

C

A

G

U

UUU (Phe/F)苯丙氨酸

UCU (Ser/S)丝氨酸

UAU (Tyr/Y)酪氨酸

UGU (Cys/C)半胱氨酸

UUC (Phe/F)苯丙氨酸

UCC (Ser/S)丝氨酸

UAC (Tyr/Y)酪氨酸

UGC (Cys/C)半胱氨酸

UUA (Leu/L)亮氨酸

UCA (Ser/S)丝氨酸

UAA (终止)

UGA (终止)

UUG (Leu/L)亮氨酸

UCG (Ser/S)丝氨酸

UAG (终止)

UGG (Trp/W)色氨酸






C

CUU (Leu/L)亮氨酸

CCU (Pro/P)脯氨酸

CAU (His/H)组氨酸

CGU (Arg/R)精氨酸

CUC (Leu/L)亮氨酸

CCC (Pro/P)脯氨酸

CAC (His/H)组氨酸

CGC (Arg/R)精氨酸

CUA (Leu/L)亮氨酸

CCA (Pro/P)脯氨酸

CAA (Gln/Q)谷氨酰胺

CGA (Arg/R)精氨酸

CUG (Leu/L)亮氨酸

CCG (Pro/P)脯氨酸

CAG (Gln/Q)谷氨酰胺

CGG (Arg/R)精氨酸






A

AUU (Ile/I)异亮氨酸

ACU (Thr/T)苏氨酸

AAU (Asn/N)天冬酰胺

AGU (Ser/S)丝氨酸

AUC (Ile/I)异亮氨酸

ACC (Thr/T)苏氨酸

AAC (Asn/N)天冬酰胺

AGC (Ser/S)丝氨酸

AUA (Ile/I)异亮氨酸

ACA (Thr/T)苏氨酸

AAA (Lys/K)赖氨酸

AGA (Arg/R)精氨酸

AUG (Met/M)甲硫氨酸

ACG (Thr/T)苏氨酸

AAG (Lys/K)赖氨酸

AGG (Arg/R)精氨酸






G

GUU (Val/V)缬氨酸

GCU (Ala/A)丙氨酸

GAU (Asp/D)天冬氨酸

GGU (Gly/G)甘氨酸

GUC (Val/V)缬氨酸

GCC (Ala/A)丙氨酸

GAC (Asp/D)天冬氨酸

GGC (Gly/G)甘氨酸

GUA (Val/V)缬氨酸

GCA (Ala/A)丙氨酸

GAA (Glu/E)谷氨酸

GGA (Gly/G)甘氨酸

GUG (Val/V)缬氨酸

GCG (Ala/A)丙氨酸

GAG (Glu/E)谷氨酸

GGG (Gly/G)甘氨酸


 

密码子偏好性表格:

 

人类密码子偏好性表格

第一位碱基

第二位碱基

第三位碱基

U

C

A

G

U

UUU17.6%

UCU15.2%

UAU12.2%

UGU10.6%

U

UUC20.3%

UCC (17.7%)

UAC15.3%

UGC (12.6%)

C

UUA7.7%

UCA12.2%

UAA1%

UGA (1.6%)

A

UUG12.9%

UCG4.4%

UAG (0.8%)

UGG (13.2%)

G

C

 

CUU (13.2%)

CCU17.5%

CAU10.9%

CGU4.5%

U

CUC 19.6%)

CCC19.8%

CAC15.1%

CGC10.4%

C

CUA (7.2%)

CCA16.9%

CAA12.3%

CGA6.2%

A

CUG39.6%

CCG6.9%

CAG34.2%

CGG11.4%

G

A

AUU16%

ACU (13.1%)

AAU17%

AGU12.1%

U

AUC (20.8%)

ACC18.9%

AAC19.1%

AGC19.5%

C

AUA7.5%)

ACA15.1%

AAA24.4%

AGA12.2%

A

AUG22%

ACG6.1%

AAG31.9%

AGG12%

G

G

GUU11%

GCU18.4%

GAU21.8%

GGU10.8%

U

GUC14.5%

GCC27.7%

GAC25.1%

GGC22.2%

C

GUA (7.1%)

GCA15.8%

GAA29%

GGA16.5%

A

GUG (28.1%)

GCG7.4%

GAG39.6%

GGG16.5%

G

 


 

大肠杆菌密码子偏好性表格

第一位碱基

第二位碱基

第三位碱基

U

C

A

G

U

UUU24.4%

UCU13.1%

UAU21.6%

UGU5.9%

U

UUC13.9%

UCC (9.7%)

UAC11.7%

UGC (5.5%)

C

UUA17.4%

UCA13.1%

UAA2%

UGA (1.1%)

A

UUG12.9%

UCG8.2%

UAG (0.3%)

UGG (13.4%)

G

C

 

CUU (14.5%)

CCU9.5%

CAU12.4%

CGU15.9%

U

CUC9.5%)

CCC6.2%

CAC7.3%

CGC14%

C

CUA (5.6%)

CCA9.1%

CAA14.4%

CGA4.8%

A

CUG37.4%

CCG14.5%

CAG26.7%

CGG7.9%

G

A

AUU29.6%

ACU (13.1%)

AAU29.3%

AGU13.2%

U

AUC (19.4%)

ACC18.9%

AAC20.3%

AGC14.3%

C

AUA13.3%)

ACA15.1%

AAA37.2%

AGA7.1%

A

AUG23.7%

ACG13.6%

AAG15.3%

AGG4%

G

G

GUU21.6%

GCU18.9%

GAU33.7%

GGU23.7%

U

GUC13.1%

GCC21.6%

GAC17.9%

GGC20.6%

C

GUA (13.1%)

GCA23%

GAA35.1%

GGA13.6%

A

GUG (19.9%)

GCG21.1%

GAG19.4%

GGG12.3%

G

 

6.2.4 空间群表格

 

6.2.5 线站新衍射仪BL17U1

简介(INTRODUCTION

上海光源BL17U1线站新衍射仪(自主研制,水平方向SOC~±0.342 μm,垂直方向SOC~±0.319 μm)和新探测器(瑞士Dectris公司的Eiger X 16 M)已经安装调试完成,1110号开始正式对用户开放使用。但是在开放初期还需要各位老师提醒实验人员注意以下事项:

1.         由于衍射仪测角头电机比较小,如果手动上样的话很容易把位置碰歪,所以目前只能机械手上样,建议各位老师将晶体样品保存在puck里,如果没有puck,可以提前到线站借用我们线站的puck将晶体样品转移到puck里再通过机械手上样。

2.         如果在实验的过程中发生故障,一定要第一时间给值班人员打电话,不能自行去处理。

3.          建议在EIGER 16 M探测器上收数据的策略是:0.1~0.3度一张,曝光时间0.05秒一张。这样收一套360度的完整数据(3600张衍射图)大概只需要3分钟。

4.         一套3600张衍射图的数据大小是25 GB左右,建议带一个存储空间大的硬盘拷贝数据,可以边实验边拷贝数据。减少拷贝数据等待时间。

5.         数据处理建议使用XDS

6.         目前线站的新系统还需要进一步优化调试,能量暂时固定在12.660 Kev

Name

Backnone

Description

Cloning Frame

Fusion Tag

Sequencing Primer 1

Sequencing Primer2

Resistance

  

  

V1

pET30a

NdeI-SalI,NotI-XhoI-His6-stop

SalI-NotI

none

T7 Promoter

T7 Terminator

Kan

  

  

V2

pET30a

NdeI-His8-SalI,NotI-stop

SalI-NotI

none

T7 Promoter

T7 Terminator

Kan

  

  

V3

pET30a

NdeI-His8-GB1-TEV-SalI,NotI-stop

SalI-NotI

His8-GB1-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V4

pET30a

NdeI-His8-GB1-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

His8-GB1-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V5

pET30a

NdeI-His8-TEV-SalI,NotI-stop

SalI-NotI

MGSS-His8-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V6

pET30a

NdeI-His8-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

MGSS-His8-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V7

pET30a

NdeI-His8-SUMO-TEV-SalI,NotI-XhoI-stop

SalI-NotI

MGSS-His8-SUMO-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V8

pET30a

NdeI-His8-VHb-TEV-SalI,NotI-stop

SalI-NotI

His8-VHb-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V9

pET30a

NdeI-His8-VHb-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

His8-VHb-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V10

pET30a

NdeI-His8-GST-TEV-SalI,NotI-stop

SalI-NotI

His8-GST-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V11

pET30a

NdeI-His8-GST-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

His8-GST-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V12

pET30a

NdeI-His7-TRX-TEV-SalI,NotI-stop

SalI-NotI

His7-TRX-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V13

pET30a

NdeI-His7-TRX-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

His7-TRX-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V14

pET30a

NdeI-His8-MBP-SalI,NotI-stop

SalI-NotI

His8-MBP

T7 Promoter

T7 Terminator

Kan

  

  

V15

pET30a

NdeI-His8-tag2-TEV-SalI,NotI-stop

SalI-NotI

His8-tag2-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V16

pET30a

NdeI-His8-tag2-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

His8-tag2-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V17

pET30a

NdeI-DsbA-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

DsbA-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V18

pET30a

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V19

pET30a

NdeI-His8-NusA-TEV-SalI,NotI-stop

SalI-NotI

His8-NusA-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V20

pET30a

NdeI-His8-GB1-TEV-SalI,NotI-Acidic   Peptide-LacZ-XhoI-His6-stop

SalI-NotI

His8-GB1-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V21

pET30a

NdeI-His8-MBP-helical linker-SalI,NotI-stop

SalI-NotI

His8-MBP-helical linker

T7 Promoter

T7 Terminator

Kan

  

  

V22

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V23

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V24

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V25

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V26

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V27

pMAL

NdeI-MBP-SalI,NotI-XhoI-LacZ-stop

SalI-NotI

MBP

M13R-pUC

M13F-pUC

Amp

  

  

V28

pET30a

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V29

pET30a

NdeI-MBP-TEV-SalI,NotI-stop

SalI-NotI

MBP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V29H

pET30a

NdeI-His8-MBP-TEV-SalI,NotI-stop

SalI-NotI

His8-MBP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V30

pET30a

NdeI-MBP-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V31

pLEXSY-Ble

NcoI-SP-SalI,NotI-His6-stop

SalI-NotI

Signal Peptide

P14

A26

Amp/Ble/Tet

  

  

V32

pLEXSY-Ble

NcoI-SP-Strep   Tag-His8-TEV-SalI,NotI-His6-stop

SalI-NotI

SP-Strep Tag-His8-TEV

P14

A26

Amp/Ble/Tet

  

  

V33

pLEXSY-Ble

NcoI-SalI,NotI-His8-stop

SalI-NotI

none

P14

A26

Amp/Ble/Tet

  

  

V34

pLEXSY-Ble

NcoI-Strep Tag-SalI,NotI-His8-stop

SalI-NotI

Strep Tag

P14

A26

Amp/Ble/Tet

  

  

V35

pLEXSY-Ble

NcoI-His8-GST-TEV-SalI,NotI-stop

SalI-NotI

His8-GST-TEV

P14

A26

Amp/Ble/Tet

  

  

V36

pLEXSY-Ble

NcoI-SP-Strep-His8-TEV-SalI,NotI-His6-stop

SalI-NotI

SP-Strep-His8-TEV

P14

A26

Amp/Ble/Tet

  

  

V37

pLEXSY-Ble

NcoI-Strep-His8-TEV-SalI,NotI-His6-stop

SalI-NotI

Strep-His8-TEV

P14

A26

Amp/Ble/Tet

  

  

V40

pTT5

NcoI-His8-GB1-TEV-SalI,NotI-stop

SalI-NotI

His8-GB1-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V41

pTT5

NcoI-His6-Strep-His8-TEV-SalI,NotI-stop

SalI-NotI

His6-Strep-His8-TEV

P14

A26

Amp/Ble/Tet

  

  

V42

pTT5

NcoI-Strep-His8-TEV-SalI,NotI-stop

SalI-NotI

Strep-His8-TEV

P14

A26

Amp/Ble/Tet

  

  

V51

pFab

EcoRV-SP-Strep-His8-TEV-SalI,NotI-His6-stop

SalI-NotI

SP-Strep-His8-TEV

unknown

unknown

Amp

  

  

V52

pFab

EcoRV-SP-His6-TEV-SalI,NotI-stop

SalI-NotI

SP-His6-TEV

unknown

unknown

Amp

  

  

V53

pFab

EcoRV-SP-His6-TEV-SalI,NotI-stop

SalI-NotI

SP-His6-TEV

unknown

unknown

Amp

  

  

V54

pFab

EcoRV-SP-His6-TEV-SalI,NotI-Strep-stop

SalI-NotI

SP-His6-TEV

unknown

unknown

Amp

  

  

V55

pMAL

NdeI-MBP-TEV-SalI,NotI-stop

SalI-NotI

MBP-TEV

M13R-pUC

M13F-pUC

Amp

  

  

V56

pMW103

LexA-SalI,NotI-stop

SalI-NotI

LexA

unknown

unknown

Kan

For Y2H

  

V57

pET30a

NdeI-hDim2-SalI,NotI-XhoI-His6-stop

SalI-NotI

hDim2

T7 Promoter

T7 Terminator

Kan

  

  

V59

pET30a

NdeI-His8-TEV-SalI,NotI-Lysozyme-stop

SalI-NotI

His8-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V60

pET30a

NdeI-Lysozyme-SalI,NotI-XhoI-His6-stop

SalI-NotI

Lysozyme

T7 Promoter

T7 Terminator

Kan

  

  

V61

pEU-01

His6-TEV-SalI,NotI-stop

SalI-NotI

His6-TEV

unknown

unknown

Amp

  

  

V62

pET30a

NdeI-MBP-SalI,NotI-lysozyme-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V63

pET30a

NdeI-MBP-TEV-SalI,NotI-Lysozyme-XhoI-His6-stop

SalI-NotI

MBP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V64

pET30a

NdeI-His8-MBP-TEV-SalI,NotI-Lysozyme-stop

SalI-NotI

His8-MBP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V65

pJG4-5

Gal1-B42-HA epitope-EcoRI-SalI,NotI-stop

SalI-NotI

Gal1-B42-HA epitope

unknown

unknown

Amp

For Y2H

  

V66

pET30a

NdeI-T4LS5-SalI,NotI-XhoI-His6-stop

SalI-NotI

T4LS5

T7 Promoter

T7 Terminator

Kan

  

  

V67

pEU-01

NdeI-His8-NcoI-TEV-SalI,NotI-stop

SalI-NotI

His8-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V71

pET30a

NdeI-SalI,NotI-XhoI-His6-stop

SalI-NotI

none

T7 Promoter

T7 Terminator

Kan

  

  

V72

pET30a

NdeI-SalI,NotI-Strep-stop

SalI-NotI

none

T7 Promoter

T7 Terminator

Kan

  

  

V77

pBAD

NdeI-SalI,NotI-XhoI-His6-stop

SalI-NotI

none

pBAD for

pBAD rev

Amp

  

  

V78

pBAD-V28E

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

pBAD for

pBAD rev

Amp

  

  

V79

pBAD-V29H

NdeI-His8-MBP-TEV-SalI,NotI-stop

SalI-NotI

His8-MBP-TEV

pBAD for

pBAD rev

Amp

  

  

V80

pCMV-HA

HAtag-EcoRI-SalI,NotI-stop

SalI-NotI

HAtag

pCMV

M13R-20

Amp

  

  

V81

pBAD-V30

NdeI-MBP-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP-TEV

pBAD for

pBAD rev

Amp

  

  

V82

pBAD-V29HS

NdeI-His8-MBP-TEV-SalI,NotI-strep-stop

SalI-NotI

His-MBP-TEV

pBAD for

pBAD rev

Amp

  

  

V83

pASK-17plus

NdeI-Strep-TEV-EcoRI-BamHI-XhoI-SalI-NcoI-EcoRV-HindIII

MCS

Strep-TEV

pASk for

pASK rev

Amp

  

  

V84

pASK-V3

NdeI-His8-GB1-TEV-SalI,NotI-stop

SalI-NotI

His8-GB1-TEV

pASk for

pASK rev

Amp

  

  

V85

pASK-V28E

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

pASk for

pASK rev

Amp

  

  

V86

pASK-V29H

NdeI-His8-MBP-TEV-SalI,NotI-stop

SalI-NotI

His-MBP-TEV

pASk for

pASK rev

Amp

  

  

V87

pASK-V30

NdeI-MBP-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP-TEV

pASk for

pASK rev

Amp

  

  

V88

pASK-V29HS

NdeI-His8-MBP-TEV-SalI,NotI-Strep-stop

SalI-NotI

His-MBP-TEV

pASk for

pASK rev

Amp

  

  

V89

pET30a

NdeI-InfB21-eGFP-SalI,NotI-stop

SalI-NotI

InfB21-eGFP

T7 Promoter

T7 Terminator

Kan

  

  

V90

pET30a

NdeI-InfB21-eGFP-TEV-SalI,NotI-stop

SalI-NotI

InfB21-eGFP

T7 Promoter

T7 Terminator

Kan

  

  

V91

pET30a

NdeI-His8-eGFP-TEV-SalI,NotI-stop

SalI-NotI

His8-eGFP-TEV

T7 Promoter

T7 Terminator

Kan

  

  

V92

pET30a

NdeI-SUMO-SalI,NotI-Strep-stop

SalI-NotI

SUMO

T7 Promoter

T7 Terminator

Kan

  

  

V93

pET30a

NdeI-His8-MBP-TEV-GFP-SalI,NotI-Strep-stop

SalI-NotI

His8-MBP-TEV-GFP

T7 Promoter

T7 Terminator

Kan

  

  

V94

pET30a

NdeI-VHb-SalI,NotI-Strep-stop

SalI-NotI

VHb

T7 Promoter

T7 Terminator

Kan

  

  

V95

pET30a

NdeI-Tag2-SalI,NotI-Strep-stop

SalI-NotI

Tag2

T7 Promoter

T7 Terminator

Kan

  

  

V96

pET30a

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V97

pET30a

NdeI-MBP-SalI,NotI-Strep-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V98

pFab

EcoRV-SP-NdeI-His8-SalI,NotI-Strep-stop

SalI-NotI

SP-His8

unknown

unknown

Amp

  

  

V99

pET30a

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V100

pET30a

NdeI-MBP-SalI,NotI-XhoI-His6-stop

SalI-NotI

MBP

T7 Promoter

T7 Terminator

Kan

  

  

V101

pET30a

NdeI-GlyA-SalI,NotI-XhoI-His6-stop

SalI-NotI

GlyA

T7 Promoter

T7 Terminator

Kan

  

  

V102

pET30a

NdeI-GacH-SalI,NotI-XhoI-His6-stop

SalI-NotI

GacH

T7 Promoter

T7 Terminator

Kan

  

  

V103

pET30a

NdeI-XBP1-SalI,NotI-XhoI-His6-stop

SalI-NotI

XBP1

T7 Promoter

T7 Terminator

Kan

  

  

V104

pGBKT7SN

DNA Binding-XhoI-NdeI-SalI,NotI-stop

SalI-NotI

DNA Binding

T7 Promoter

ADH

Kan

  

  

V105

pGADT7SN

SV40-AD-NcoI-Epitope-NdeI-SalI,NotI-stop

SalI-NotI

SV40-AD-Epitope

T7 Promoter

ADH

Amp

  

  

V106

pRmHa3

EcoRI-TEV-SalI,NotI-XhoI-His6-stop

SalI-NotI

TEV

pRmHa3 for

pRmHa3 rev

Amp

  

  

V107

pVL1392

NcoI-NotI-HIs6-stop

unknown

none

y326 polyhedrin

y327 polyhedrin

Amp

  

  

V108

plSUMOstar

HIs6-SUMOstar-SalI,NotI-stop

SalI-NotI

SUMOstar

unknown

unknown

Amp/Get

  

  

V109

pFastBacHT

NdeI-InfB21-His8-TEV-SalI,NotI-stop

SalI-NotI

InfB21-His8-TEV

pASk for

pASK rev

Amp/Get

  

  

V110

pFastBacHT

NdeI-SalI,NotI-XhoI-His6-stop

SalI-NotI

none

pASk for

pASK rev

Amp/Get

  

  

V111

pFastBacHT

NdeI-His8-MBP-TEV-Sali,NotI-stop

SalI-NotI

His8-MBP-TEV

pASk for

pASK rev

Amp/Get

  

  

V112

pFastBacHT

NdeI-His8-MBP-TEV-Sali,NotI-XhoI-His6-stop

SalI-NotI

His8-MBP-TEV

pASk for

pASK rev

Amp/Get

  

  

V113

pColdII

NdeI-His8-MBP-TEV-SalI,NotI-stop

SalI-NotI

His8-MBP-TEV

pCold for

pCold rev

Amp

  

  

V114

pColdII

NdeI-MBP-TEV-SalI,NotI-stop

SalI-NotI

MBP-TEV

pCold for

pCold rev

Amp

  

  

V115

pColdII

NdeI-XhoI-His6-stop

unknown

none

pCold for

pCold rev

Amp

  

  

V116

pET30a

NdeI-B562-XhoI-His6-stop

unknown

B562

T7 Promoter

T7 Terminator

Kan

  

  

V117

pCold

  

  

  

  

  

  

  

  

V118

pGADzaA

  

  

  

  

  

  

  

  

V119

FBQH

His8-TEV-BamHI-XhoI

BamHI-XhoI

His8-TEV

FBQ-F

FBQ-R

Amp

  

  

V120

FBQH

GST-TEV-BamHI-Xho I

BamHI-XhoI

GST-TEV

FBQ-F

FBQ-R

Amp

  

  

V121

FBQH

His-MBP-TEV-BamHI-XhoI

BamHI-XhoI

His-MBP-TEV

FBQ-F

FBQ-R

Amp

  

  

V122

PET30a

NdeI- His8- GB1 -Sal I -Not I-stop

Sal I-Not I

His8- GB1

T7-P

T7-T

Kan

XY

  

V123

pCDNA3.1

CMV-T7-FLAG-MCS-Stop

XhoI-BamH I-EcoR I-Not I

FLAG

T7/CMV

BGH-R

Amp

XY

  

V124

pCDNA3.1

CMV-T7-HA-MCS-Stop

XhoI-BamH I-EcoR I-Not I

HA

T7/CMV

BGH-R

Amp

XY

  

V125

pCDNA3.1

CMV-T7-Strep-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Strep

T7/CMV

BGH-R

Amp

XY

  

V126

pCDNA3.1

CMV-T7-SP-3XFLAG-MCS-Stop

XhoI-BamH I-EcoR I-Not I

3XFLAG

T7/CMV

BGH-R

Amp

XY

SP=signal peptide

V127

pCDNA3.1

CMV-T7-SP-FLAG-Clover-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Clover/FLAG

T7/CMV

BGH-R

Amp

XY

SP=signal peptide

V128

pCDNA3.1

CMV-T7-SP-HA-MCS-Stop

XhoI-BamH I-EcoR I-Not I

HA

T7/CMV

BGH-R

Amp

XY

SP=signal peptide

V129

pCDNA3.1

CMV-T7-SP-Strep-mRubby2-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Strep/mRubby2

T7/CMV

BGH-R

Amp

XY

SP=signal peptide

V130

pCDNA3.1

CMV-T7-SP-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Strep

T7/CMV

BGH-R

Amp

XY

  

V131

pCDNA3.1

CMV-T7-Strep-mRubby2-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Strep/mRubby2

T7/CMV

BGH-R

Amp

XY

  

V132

pCDNA3.1

CMV-T7-FLAG-Clover-MCS-Stop

XhoI-BamH I-EcoR I-Not I

Clover/FLAG

T7/CMV

BGH-R

Amp

XY

  

V133

pCDNA3.1

CMV-T7-Strep-NEGFP-MCS-Stop

XhoI-Not I

Strep/NEGFP

T7/CMV

BGH-R

Amp

XY

  

V134

pCDNA3.1

CMV-T7-MCS-CEGFP-Myc-Stop

XhoI-Not I

Myc/CEGFP

T7/CMV

BGH-R

Amp

XY

  

V135

PRSF

T7-His6-MCS-Stop/T7-MBP-TEV-MCS-His6-Stop

BamH I-Not I/Hind III-EcoR I

His/MBP

Z21

Z20

Kan

XY

  

V136

plsecSUMOstar

GP67A-HIs6-SUMOstar-SalI,NotI-stop

Sal I-Not I

His

Y935

FXJ57

Amp

  

  

V137

plsecSUMOstar

GP67A-HIs6-SUMOstar-MBP-TEV-SalI,NotI-stop

Sal I-Not I

His/MBP

Z8

FXJ57

Amp

  

  

V138

plsecSUMOstar

GP67A-HIs6-TEV-SalI,NotI-stop

Sal I-Not I

His-TEV

Z8

FXJ57

Amp

  

  

V139

plsecSUMOstar

GP67A-HIs6-SalI,NotI-stop

Sal I-Not I

His

FXJ66

  

Amp

  

  

V140

PTT5

IFNA1-SalI,XbaI-His6-stop

Sal I-Xba I

His

PTT5-F

PTT5-R

Amp

  

  

V141

PTT5

IFNA1-SalI,XbaI-Fc-stop

Sal I-Xba I

Fc

PTT5-F

PTT5-R

Amp

  

  

V142

PTT5

IFNA1-SalI,XbaI-Fc-TEV-stop

Sal I-Xba I

Fc-TEV

PTT5-F

PTT5-R

Amp

  

  

V143

plsecSUMOstar

GP67A-HIs6-SUMOstar-EGFP-TEV-SalI,NotI-stop

Sal I-Not I

His-EGFP-TEV

Z8

FXJ57

Amp

  

  

 


1.    DH5α菌株

基因型:F-φ80dlacZΔM15ΔlacZYA-argFU169deoRrecA1endA1hsdR17rk-

        mk+)、phoAsupE44λ-thi-1gyrA96relA1

DH5α是一种常用于质粒克隆的菌株。E.coli DH5α在使用pUC系列质粒载体转化时,其Φ80dlacZΔM15基因的表达产物与pUC载体编码的β-半乳糖苷酶氨基端实现α互补,可用于蓝白斑筛选。recA1endA1的突变有利于克隆DNA的稳定和高纯度质粒DNA的提取。用于分子克隆、质粒提取和蛋白质表达。

2.    TOP10菌株

基因型:F- mcrAΔ(mrr-hsd RMS-mcrBC) φ80lacZΔM15lac74 recA1araΔ139Δ(ara-leu)7697galUgalKrps(Strr) endA1nupG

该菌株适用于高效的DNA克隆和质粒扩增,能保证高拷贝质粒的稳定遗传。

3.    HB101菌株

基因型:supE44hsdS20rB-mB-)、recA13ara14proA2lacY1galK2rpsL20xyl-5mtl-1leuB6thi-1。该菌株遗传性能稳定,使用方便,适用于各种基因重组实验。

4.    XL1-Blue菌株

基因型:endA1gyrA96(nalR)thi-1recA1relA1lacglnV44F[Tn10 proAB+ lacIq Δ(lacZ)M15]hsdR17(rK- mK+)。该菌株具有卡那抗性、四环素抗性和氯霉素抗性,用于分子克隆和质粒提取。

5.    DH10B菌株

基因型:F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1deoRΔ(ara,leu)7697 araD139galUgalKnupGrpsLλ-

该菌株消除了mcrAmcrBCmrrhsdRMS限制系统,允许构建更多具有代表性的基因组文库。endA1突变,可以增加质粒产量和数量。高效率转化大小为150 kb的质粒,用于产生cDNA或基因组文库。lacZΔM15 用于重组克隆的蓝/白斑筛选。

6.    B834菌株

BL21菌株的父代菌株。适用于用Se-Met方法表达硒代蛋白。无抗性。

7.    BL21(DE3)菌株

基因型:F-ompThsdSrBB-mB-)、galdcmDE3

该菌株用于高效表达克隆于含有噬菌体T7启动子的表达载体(如pET系列)的基因。T7噬菌体RNA聚合酶位于λ 噬菌体DE3区,该区整合于BL21的染色体上。该菌适合表达非毒性蛋白。

8.    BL21(DE3) pLysS菌株

基因型:F-ompT hsdSrBB-mB-)、galdcmDE3)、pLysSCamr

该菌株含有质粒pLysS,因此具有氯霉素抗性。PLysS含有表达T7溶菌酶的基因,能够通过直接与T7 RNA 聚合酶结合并抑制其活性来降低目的基因的背景表达水平,但不干扰IPTG诱导的表达。该菌适合表达毒性蛋白和非毒性蛋白。需要用更高浓度IPTG比如0.7 mM来诱导。

9.    BL21(DE3) RosettaTM菌株(Novagen)

GenotypeF-ompThsdSB(rB- mB-)galdcm (DE3)pRARE (CamR)。该菌株是BL21额外含有一个编码稀有密码子的质粒,因此具有氯霉素抗性。

   Rosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.

10.  BL21 Codon plusTM(DE3)-RIPL (Stratagene)

    Antibiotic ResistanceCamr Strep/Specr 该菌株是BL21额外含有一个编码稀有密码子的质粒,因此具有氯霉素抗性。

    BL21-CodonPlus competent cells are derived from the high-performance Stratagene BL21-Gold competent cell line. These cells enable efficient high-level expression of heterologous proteins in Escherichia coli.
   BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. Availability of tRNAs allows high-level expression of many heterologous recombinant genes in BL21-CodonPlus cells that are poorly expressed in conventional BL21 strains. The BL21-CodonPlus (DE3)-RIPL cells contain extra copies of the argU, ileY, and leuW as well as the + tRNA genes. This strain rescues expression of heterologous proteins from organisms that have either AT- or GC-rich genomes

11.  JM109菌株

基因型:recA1endA1gyrA96thi1hsdR17supE44relA1ΔlacproAB/F’[traD36proAB+lacIqlacZΔM15]。该菌株在使用pUC系列质粒载体进行DNA转化或用M13 phage载体进行转染时,由于载体DNA产生的LacZa多肽和JM09编码的LacZΔM15进行α-互补,从而显示β-半乳糖苷酶活性,由此很容易鉴别重组体菌株。部分抗性缺陷,适合重复基因表达, 可用于M13克隆序列测定和蓝白斑筛选。用于分子克隆、质粒提取和蛋白质表达。

12.  293T细胞

293T细胞来源都是人胚胎肾细胞,通过基因技术派生的细胞系,经过腺病毒E1A基因转染,能表达SV40T抗原,含有SV40复制起始点与启动子区。许多真核表达载体如pcDNA3.1中含有SV40病毒的复制起始位点,可在表达SV40病毒T抗原的细胞系中复制,从而提高外源基因的表达水平。因此293T细胞广泛用于病毒包装。用磷酸钙转染效率可高达50%。蛋白表达水平高,转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬转293T细胞是过表达蛋白并获得细胞内及细胞外(分泌的或膜)蛋白的便捷方式。

 

Table of Cell Strains

Table Cell strains

 

 

Cell Strains

Resistance

Usage

1

Sf9

none

包装病毒

2

Hi5

none

昆虫细胞表达蛋白

3

HEK293

none

表达包装病毒

4

293T

none

工具细胞

5

L929

none

分泌gmcsf,促进巨噬细胞分化

 

 

 

 

 

 

 

 


 

Table of Bacteria Strains

Table Bacteria Strains

 

 

Bacteria Strains

Resistance

Usage

1

DH5α

none

Plasmid transformation

2

Top 10

none

Plasmid transformation

3

TOP 10 F’

Tet

Plasmid transformation

4

XL1blue MRF’ Kan

Kan

Plasmid transformation

5

xl-10 gold

Tet & Chl

Plasmid transformation

6

ccdB survival 2 T1R,   Invitrogen

Sm

Plasmid transformation

7

Survival-2

Sm

Plasmid transformation

8

Ex0-DH5α  Amp

No found

Plasmid transformation

9

Lig-DH5α  Amp

No found

Plasmid transformation

10

BL21(DE3)

none

Protein expression

11

BL21(DE3), pLysS

Chl

Protein expression

12

BL21*

none

Protein expression

13

BL21/AI  Invitrogen

Tet

Protein expression

15

BL21,RIL,RIPL

Chl

Protein expression

16

Origami B

Kan & Tet

Protein expression

17

Rosetta Gami

Kan & Tet & Chl

Protein expression

18

Tuner

none

Protein expression

19

B834

none

Protein expression

20

55244

Kan

Protein expression

21

Tuvp20

No found

 

22

AH109 (Yeast)

none

酿酒酵母,酵母双杂交,蛋白与蛋白互作

23

SMD1168H (Yeast)

none

毕赤酵母,蛋白表达

24

CG1945 (Yeast)

none

酿酒酵母,酵母双杂交,蛋白与蛋白互作,需要mating

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 















        表格中”No found” 部分因未查到其细胞抗性,建议实验中视为无抗性处理,其具体特性请自行查询。

 


1.2.8 结晶试剂盒


  简介(INTRODUCTION

长晶体是由清澈的蛋白质溶液变成饱和溶液,饱和溶液变成过饱和溶液,过饱和溶液如果满足适当的条件时蛋白质有可能以晶体形式从溶液中析出,否则就会变成无定形沉淀。而结晶过程不可能自发地完成,必须要在外界的帮助下才可能完成这种熵减的过程。





我们实验室用的是悬滴气象扩散法,在起始状态时,结晶母液与外液之间存在某些因素的梯度差,随着气相扩散,这些梯度发生变化。如果变化后的条件合适,母液中的蛋白质就有可能以晶体状态析出。


我们实验室有很多长晶体试剂盒,还有一个金老师自己研制出来的MBP-screen。原则上带MBP标签的蛋白一开始可以用MBP-screen来筛选,如果没有晶体长出来时,根据蛋白在该试剂盒的表现(都是沉淀还是都是清澈的液滴)来决定下一步用什么试剂盒。本附录中收纳了我们实验室已有的所有试剂盒的配方。


实验室现有晶体试剂盒介绍

1.  MBP-phusion Protein Crystallization Screen

本实验室研制,利用率和成功率最高的试剂盒。是MBP偶联蛋白的首选,其他样品也适用,一般用于初筛。

Emerald BioSystems

2.   Wizard™ I/random sparse matrix crystallization screen

3.   Wizard™ III/random sparse matrix crystallization screen

适合初筛,pH值横跨4.5-10.5,不同的buffer,不同的盐,沉淀剂也有很多种。

4.    The Anions Suite

该试剂盒组分都是盐离子,没有以PEGEG或者甘油作为沉淀剂。如果蛋白在初筛的过程中,以PEG

沉淀剂的情况下极其容易沉淀时,可以用该试剂盒试一下。

5.    The Nucleix Suite

相对来说是核酸-蛋白复合物的首选试剂盒。

6.    The PACT Suite

以不同的PEG为沉淀剂的试剂盒,如果初筛后发现你的蛋白在以PEG为沉淀剂(PEG 1000以上)的情况下长晶体的可能性较大时,这个试剂盒可以帮你确定哪个PEG搭配哪个盐离子比较好。

7.   The Protein Complex Suite

顾名思义,适合蛋白复合物的晶体筛选,但同时也适合来自哺乳动物的蛋白。

8.   Natrix HTHR2-131

主要用于核酸以及核酸-蛋白质复合物

9.    SaltRx HTHR2-136

该试剂盒与QiagenAnions试剂盒相似,主要成分全部都是盐离子,其中包含22中盐离子,配与不同的浓度和pH环境,在初筛中发现蛋白倾向于在盐离子环境下长晶体时可以考虑这个试剂盒。

10.  PEGRx HTHR2-086

用于生物大分子的晶体筛选,其实也是比较适合用于初筛。

11.  Crystal Screen HTHR2-130

该试剂盒是非常经典的一款试剂盒,里面包含不同的沉淀剂,不同的盐离子以及不同的pH环境,非常适合初筛。


 

Wizard™ I random sparse matrix crystallization screen

No.

crystallant

salt (0.2 M)

buffer   (0.1 M)

1

20%   (w/v) PEG-8000

none

CHES pH 9.5

2

10%   (v/v) 2-propanol

NaCl

HEPES pH 7.5

3

15%   (v/v) ethanol

none

CHES pH 9.5

4

35%   (v/v) 2-methyl-2,4-pentanediol

MgCl2

imidazole pH   8.0

5

30%   (v/v) PEG-400

none

CAPS pH 10.5

6

20%   (w/v) PEG-3000

none

citrate pH 5.5

7

10%   (w/v) PEG-8000

Zn(OAc)2

MES pH 6.0

8

2.0   M (NH4)2SO4

none

citrate pH 5.5

9

1.0   M (NH4)2HPO4

none

acetate pH 4.5

10

20%   (w/v) PEG-2000 MME

none

Tris pH 7.0

11

20%   (v/v) 1,4-butanediol

Li2SO4

MES pH 6.0

12

20%   (w/v) PEG-1000

Ca(OAc)2

imidazole pH   8.0

13

1.26   M (NH4)2SO4

none

cacodylate pH   6.5

14

1.0   M sodium citrate

none

cacodylate pH   6.5

15

10%   (w/v) PEG-3000

Li2SO4

imidazole pH   8.0

16

2.5   M NaCl

none

Na/K phosphate   pH 6.2

17

30%   (w/v) PEG-8000

Li2SO4

acetate pH 4.5

18

1.0   M K/Na tartrate

NaCl

imidazole pH   8.0

19

20%   (w/v) PEG-1000

none

Tris pH 7.0

20

0.4   M NaH2PO4/1.6 M K2HPO4

NaCl

imidazole pH   8.0

21

20%   (w/v) PEG-8000

none

HEPES pH 7.5

22

10%   (v/v) 2-propanol

none

Tris pH 8.5

23

15%   (v/v) ethanol

MgCl2

imidazole pH   8.0

24

35%   (v/v) 2-methyl-2,4-pentanediol

NaCl

Tris pH 7.0

25

30%   (v/v) PEG-400

MgCl2

Tris pH 8.5

26

10%   (w/v) PEG-3000

none

CHES pH 9.5

27

1.2   M NaH2PO4/0.8 M K2HPO4

Li2SO4

CAPS pH 10.5

28

20%   (w/v) PEG-3000

NaCl

HEPES pH 7.5

29

10%   (w/v) PEG-8000

NaCl

CHES pH 9.5

30

1.26   M (NH4)2SO4

NaCl

acetate pH 4.5

31

20%   (w/v) PEG-8000

NaCl

phosphate-citrate   pH 4.2

32

10%   (w/v) PEG-3000

none

Na/K phosphate   pH 6.2

33

2.0   M (NH4)2SO4

Li2SO4

CAPS pH 10.5

34

1.0   M (NH4)2HPO4

none

imidazole pH   8.0

35

20%   (v/v) 1,4-butanediol

none

acetate pH 4.5

36

1.0   M sodium citrate

none

imidazole pH   8.0

37

2.5   M NaCl

none

imidazole pH   8.0

38

1.0   M K/Na tartrate

Li2SO4

CHES pH 9.5

39

20%   (w/v) PEG-1000

Li2SO4

phosphate-citrate   pH 4.2

40

10%   (v/v) 2-propanol

Ca(OAc)2

MES pH 6.0

41

30%   (w/v) PEG-3000

none

CHES pH 9.5

42

15%   (v/v) ethanol

none

Tris pH 7.0

43

35%   (v/v) 2-methyl-2,4-pentanediol

none

Na/K phosphate   pH 6.2

44

30%   (v/v) PEG-400

Ca(OAc)2

acetate pH 4.5

45

20%   (w/v) PEG-3000

none

acetate pH 4.5

46

10%   (w/v) PEG-8000

Ca(OAc)2

imidazole pH   8.0

47

1.26   M (NH4)2SO4

Li2SO4

Tris pH 8.5

48

20%   (w/v) PEG-1000

Zn(OAc)2

acetate pH 4.5


Wizard™ I random sparse matrix crystallization screen

No.

crystallant

salt (0.2 M)

buffer   (0.1 M)

49

10%   (w/v) PEG-3000

Zn(OAc)2

acetate pH 4.5

50

35%   (v/v) 2-methyl-2,4-pentanediol

Li2SO4

MES pH 6.0

51

20%   (w/v) PEG-8000

MgCl2

Tris pH 8.5

52

2.0   M (NH4)2SO4

NaCl

cacodylate pH   6.5

53

20%   (v/v) 1,4-butanediol

NaCl

HEPES pH 7.5

54

10%   (v/v) 2-propanol

Li2SO4

phosphate-citrate   pH 4.2

55

30%   (w/v) PEG-3000

NaCl

Tris pH 7.0

56

10%   (w/v) PEG-8000

NaCl

Na/K phosphate   pH 6.2

57

2.0   M (NH4)2SO4

none

phosphate-citrate   pH 4.2

58

1.0   M (NH4)2HPO4

none

Tris pH 8.5

59

10%   (v/v) 2-propanol

Zn(OAc)2

cacodylate pH   6.5

60

30%   (v/v) PEG-400

Li2SO4

cacodylate pH   6.5

61

15%   (v/v) ethanol

Li2SO4

citrate pH 5.5

62

20%   (w/v) PEG-1000

NaCl

Na/K phosphate   pH 6.2

63

1.26   M (NH4)2SO4

none

HEPES pH 7.5

64

1.0   M sodium citrate

none

CHES pH 9.5

65

2.5   M NaCl

MgCl2

Tris pH 7.0

66

20%   (w/v) PEG-3000

Ca(OAc)2

Tris pH 7.0

67

1.6   M NaH2PO4/0.4 M K2HPO4

none

phosphate-citrate   pH 4.2

68

15%   (v/v) ethanol

Zn(OAc)2

MES pH 6.0

69

35%   (v/v) 2-methyl-2,4-pentanediol

none

acetate pH 4.5

70

10%   (v/v) 2-propanol

none

imidazole pH   8.0

71

15%   (v/v) ethanol

MgCl2

HEPES pH 7.5

72

30%   (w/v) PEG-8000

NaCl

imidazole pH   8.0

73

35%   (v/v) 2-methyl-2,4-pentanediol

NaCl

HEPES pH 7.5

74

30%   (v/v) PEG-400

none

CHES pH 9.5

75

10%   (w/v) PEG-3000

MgCl2

cacodylate pH   6.5

76

20%   (w/v) PEG-8000

Ca(OAc)2

MES pH 6.0

77

1.26   M (NH4)2SO4

NaCl

CHES pH 9.5

78

20%   (v/v) 1,4-butanediol

Zn(OAc)2

imidazole pH   8.0

79

1.0   M sodium citrate

NaCl

Tris pH 7.0

80

20%   (w/v) PEG-1000

none

Tris pH 8.5

81

1.0   M (NH4)2HPO4

NaCl

citrate pH 5.5

82

10%   (w/v) PEG-8000

none

imidazole pH   8.0

83

0.8   M NaH2PO4/1.2 M K2HPO4

none

acetate pH 4.5

84

10%   (w/v) PEG-3000

NaCl

phosphate-citrate   pH 4.2

85

1.0   M K/Na tartrate

Li2SO4

Tris pH 7.0

86

2.5   M NaCl

Li2SO4

acetate pH 4.5

87

20%   (w/v) PEG-8000

NaCl

CAPS pH 10.5

88

20%   (w/v) PEG-3000

Zn(OAc)2

imidazole pH   8.0

89

2.0   M (NH4)2SO4

Li2SO4

Tris pH 7.0

90

30%   (v/v) PEG-400

NaCl

HEPES pH 7.5

91

10%   (w/v) PEG-8000

MgCl2

Tris pH 7.0

92

20%   (w/v) PEG-1000

MgCl2

cacodylate pH 6.5

93

1.26   M (NH4)2SO4

none

MES pH 6.0

94

1.0   M (NH4)2HPO4

NaCl

imidazole pH   8.0

95

2.5   M NaCl

Zn(OAc)2

imidazole pH   8.0

96

1.0   M K/Na tartrate

none

MES pH 6.0


Wizard™ III random sparse matrix crystallization screen

No

crystallant

salt/additive #1

additive #2

buffer

pH

1

20%(w/v)   PEG 3350

0.2   M ammonium citrate (dibasic)

 

 

 

2

30%(v/v)   MPD

0.02   M calcium chloride

 

0.1   M sodium acetate

4.6

3

20%(w/v)   PEG 3350

0.2   M magnesium formate

 

 

 

4

20%(w/v)   PEG 3350

0.2   M ammonium formate

 

 

 

5

20%(w/v)   PEG 3350

0.2   M ammonium chloride

 

 

 

6

20%(w/v)   PEG 3350

0.2   M potassium formate

 

 

 

7

50%(w/v)   MPD

0.2   M NH4H2PO4

 

0.1   M Tris

8

8

20%(w/v)   PEG 3350

0.2   M potassium nitrate




9

0.8   M ammonium sulfate



0.1   M citric acid

4.0

10

20%(w/v)   PEG 3350

0.2   M sodium thiocyanate




11

20%(w/v)   PEG 6000



0.1   M bicine

9.0

12

10%(w/v)   PEG 8000

8%(v/v)   ethylene glycol


0.1   M HEPES

7.5

13

8%(w/v)   PEG 4000



0.1   M sodium acetate

4.6

14

20%(w/v)   PEG 6000



0.1   M citric acid

5.0

15

1.6   M Sodium citrate





16

20%(w/v)   PEG 3350

0.2   M potassium citrate tribasic




17

20%(w/v)   PEG 4000

10%(v/v)   2-propanol


0.1   M citrate

5.5

18

20%(w/v)   PEG 6000

1   M lithium chloride


0.1   M citric acid

4.0

19

20%(w/v)   PEG 3350

0.2   M ammonium nitrate




20

10%(w/v)   PEG 6000



0.1   M HEPES

7.0

21

1.6   M Na/K phosphate



0.1   M HEPES

7.5

22

20%(v/v)   ethanol



0.1   M Tris

8.5

23

10%(w/v)   PEG 20,000

2%(v/v)   dioxane


0.1   M bicine

9.0

24

2   M ammonium sulfate



0.1   M sodium acetate

4.6

25

10%(w/v)   PEG 1000

10%(w/v)   PEG 8000




26

24%(w/v)   PEG 1500

20%(v/v)   glycerol




27

30%(v/v)   PEG 400

0.2   M magnesium chloride


0.1   M HEPES

7.5

28

70%(v/v)   MPD



0.1   M HEPES

7.5

29

40%(v/v)   MPD



0.1   M Tris

8.0

30

5.5%(w/v)   PEG 4000

0.17   M ammonium sulfate

15%(v/v)   glycerol



31

14%(v/v)   2-propanol

0.14   M calcium chloride

30%(v/v)   glycerol

0.07   M sodium acetate

4.6

32

16%(w/v)   PEG 8000

0.04   M KH2PO4

20%(v/v)   glycerol



33

1.6   M magnesium sulfate



0.1   M MES

6.5

34

10%(w/v)   PEG 6000



0.1   M bicine

9.0

35

4.4%(w/v)   PEG 8000

0.16   M calcium acetate

20%(v/v)   glycerol

0.08   M cacodylate

6.5

36

30%(v/v)   Jeffamine M-600

0.05   M cesium chloride


0.1   M MES

6.5

37

3.2   M ammonium sulfate



0.1   M citric acid

5.0

38

15%(w/v)   PEG 10,000

2%(v/v)   dioxane


0.1   M citrate

5.5

39

20%(v/v)   Jeffamine M-600



0.1   M HEPES

7.5

40

10%(v/v)   MPD



0.1   M bicine

9.0

41

28%(v/v)   PEG 400

0.2   M calcium chloride


0.1   M HEPES

7.5

42

30%(w/v)   PEG 4000

0.2   M lithium sulfate


0.1   M Tris

8.5

43

30%(w/v)   PEG 8000

0.2   M ammonium sulfate




44

30%(w/v)   PEG 5000 MME

0.2   M lithium sulfate


0.1   M Tris

8.0

45

1.5   M ammonium sulfate


12%(v/v)   glycerol

0.1   M Tris

8.5

46

50%(v/v)   MPD

0.2   M NH4H2PO4

 

0.1   M Tris

8.5

47

30%(w/v)   PEG 5000 MME

0.2   M ammonium sulfate

 

0.1   M MES

6.5

48

20%(w/v)   PEG 10,000

 

 

 

7.5

Wizard™ III random sparse matrix crystallization screen

 

No

crystallant

salt/additive   #1

additive   #2

buffer

pH

49

20%(v/v) Glycerol

40 mM Potassium phosphate

16%(w/v) PEG 8000



50

15%(v/v) Ethanol

100 mM Sodium chloride

5%(v/v) MPD

Tris

8.0

51

40%(v/v) Ethanol


5%(w/v) PEG 1000

Phosphate-citrate

4.2

52

200 mM Ammonium sulfate



BisTris

5.5

53

2 M Ammonium sulfate


2%(v/v) PEG 400

Acetate

5.5

54

800 mM Ammonium Sulfate



Citrate

4.0

55

2 M Lithium sulfate

100 mM Magnesium sulfate

5%(v/v) Isopropanol

Acetate

4.5

56

2 M Lithium sulfate


2%(v/v) PEG 400

Tris

8.5

57

2 M Lithium sulfate

100 mM Magnesium sulfate

5%(v/v) PEG 400

Acetate

5.5

58

50%(v/v) PEG 200

200 mM Magnesium chloride


Na Cacodylate

6.5

59

40%(v/v) PEG 300

200 mM Calcium acetate


Na Cacodylate

6.5

60

30%(v/v) Jeffamine M600 pH 7.0

200 mM Lithium sulfate


HEPES

7.0

61

800 mM Succinic Acid pH 7.0





62

40%(v/v) PEG 400

200 mM Lithium sulfate


Tris

8.5

63

50%(v/v) PEG 400

200 mM Lithium sulfate


Acetate

4.5

64

15%(v/v) PEG 550 MME



MES

6.5

65

25%(w/v) PEG 1500



SPG Buffer/NaOH

5.5

66

25%(w/v) PEG 1500



SPG Buffer/NaOH

8.5

67

25%(w/v) PEG 1500



MMT Buffer/NaOH

6.5

68

25%(w/v) PEG 1500



MMT Buffer/NaOH

9.0

69

25%(w/v) PEG 1500



MIB Buffer/HCl

5.0

70

25%(w/v) PEG 1500



PCB Buffer/NaOH

7.0

71

12%(w/v) PEG 1500

2500 mM Sodium chloride

1.5%(v/v) MPD

Na Acetate

5.5

72

2400 mM Sodium Malonate





73

30%(w/v) PEG 2000 MME

150 mM Potassium bromide




74

10%(w/v) PEG 2000 MME

200 mM Ammonium sulfate


Sodium acetate

5.5

75

20%(w/v) PEG 2000 MME

200 mM Trimethylamine n-oxide

Tris

8.5

76

20%(w/v) PEG 3350

200 mM Sodium fluoride


BisTris Propane

6.5

77

20%(w/v) PEG 3350

200 mM Sodium citrate


Citrate

4.0

78

20%(w/v) PEG 3350

200 mM Sodium malonate


BisTris Propane

8.5

79

20%(w/v) Polyacrylic acid 5100

20 mM Magnesium chloride


HEPES

7.0

80

2100 mM DL Malic acid pH 7.0





81

800 mM K2HPO4


800 mM Na3PO4

HEPES

7.5

82

20%(w/v) PEG 6000

200 mM Ammonium chloride


MES

6.0

83

20%(w/v) PEG 6000

200 mM Sodium chloride


HEPES

7.0

84

20%(w/v) PEG 6000

200 mM Lithium chloride


Tris

8.0

85

20%(w/v) Polyvinylpyrrolidone K15

100 mM Cobalt chloride


Tris

8.5

86

50%(v/v) Ethylene glycol

200 mM Magnesium chloride


Tris

8.5

87

20%(w/v) PEG 8000


3%(v/v) MPD

Imidazole

6.5

88

20%(w/v) PEG 8000

100 mM Magnesium chloride

20%(v/v) PEG 400

Tris

8.5

89

20%(w/v) PEG 8000

200 mM Ammonium sulfate

10%(v/v) Isopropanol

HEPES

7.5

90

30%(v/v) MPD


25%(w/v) PEG 1500

Acetate

4.5

91

30%(v/v) MPD

200 mM Ammonium sulfate

10%(w/v) PEG 3350

Imidazole

6.5

92

30%(v/v) MPD

500 mM Sodium chloride

8%(w/v) PEG 8000

Tris

8.5

93

40%(v/v) Isopropanol


15%(w/v) PEG 8000

Imidazole-cl

6.5

94

30%(v/v) Isopropanol


30%(w/v) PEG 3350

Tris

8.5

95

17%(w/v) PEG 10000

100 mM Ammonium Acetate


BisTris

5.5

96

15%(w/v) PEG 20000



HEPES

7.0


The Anions Suite

No

Precipitant

Salt

Buffer   

1

2.5   M Sodium acetate


0.1 M Sodium   acetate pH 4.6

2

1.25   M Sodium acetate


0.1 M Sodium   acetate pH 4.6

3

1.2   M tri-Sodium citrate


0.1 M Sodium   acetate pH 4.6

4

0.6   M tri-Sodium citrate


0.1 M Sodium   acetate pH 4.6

5

0.6   M Sodium fluoride


0.1 M Sodium   acetate pH 4.6

6

0.3   M Sodium fluoride


0.1 M Sodium   acetate pH 4.6

7

2.5   M Sodium acetate


0.1 M MES pH   6.5

8

1.25   M Sodium acetate


0.1 M MES pH   6.5

9

1.2   M tri-Sodium citrate


0.1 M MES pH   6.5

10

0.6   M tri-Sodium citrate


0.1 M MES pH   6.5

11

0.6   M Sodium fluoride


0.1 M MES pH   6.5

12

0.3   M Sodium fluoride


0.1 M MES pH   6.5

13

2.5   M Sodium acetate


0.1 M HEPES pH   7.5

14

1.25   M Sodium acetate


0.1 M HEPES pH   7.5

15

1.2   M tri-Sodium citrate


0.1 M HEPES pH   7.5

16

0.6   M tri-Sodium citrate


0.1 M HEPES pH   7.5

17

0.6   M Sodium fluoride


0.1 M HEPES pH   7.5

18

0.3   M Sodium fluoride


0.1 M HEPES pH   7.5

19

2.5   M Sodium acetate


0.1 M TRIS.HCl   pH 8.5

20

1.25   M Sodium acetate


0.1 M TRIS.HCl   pH 8.5

21

1.2   M tri-Sodium citrate


0.1 M TRIS.HCl   pH 8.5

22

0.6   M tri-Sodium citrate


0.1 M TRIS.HCl   pH 8.5

23

0.6   M Sodium fluoride


0.1 M TRIS.HCl   pH 8.5

24

0.3   M Sodium fluoride


0.1 M TRIS.HCl   pH 8.5

25

3.5   M Sodium formate


0.1 M Sodium   acetate pH 4.6

26

1.75   M Sodium formate


0.1 M Sodium   acetate pH 4.6

27

3.5   M Sodium bromide


0.1 M Sodium   acetate pH 4.6

28

1.75   M Sodium bromide


0.1 M Sodium   acetate pH 4.6

29

2.4   M Sodium malonate


0.1 M Sodium   acetate pH 4.6

30

1.2   M Sodium malonate


0.1 M Sodium   acetate pH 4.6

31

3.5   M Sodium formate


0.1 M MES pH   6.5

32

1.75   M Sodium formate


0.1 M MES pH   6.5

33

3.5   M Sodium bromide


0.1 M MES pH   6.5

34

1.75   M Sodium bromide


0.1 M MES pH   6.5

35

2.4   M Sodium malonate


0.1 M MES pH   6.5

36

1.2   M Sodium malonate


0.1 M MES pH   6.5

37

3.5   M Sodium formate


0.1 M HEPES pH   7.5

38

1.75   M Sodium formate


0.1 M HEPES pH   7.5

39

3.5   M Sodium bromide


0.1 M HEPES pH   7.5

40

1.75   M Sodium bromide


0.1 M HEPES pH   7.5

41

2.4   M Sodium malonate


0.1 M HEPES pH   7.5

42

1.2   M Sodium malonate


0.1 M HEPES pH   7.5

43

3.5   M Sodium formate


0.1 M TRIS.HCl   pH 8.5

44

1.75   M Sodium formate


0.1 M TRIS.HCl   pH 8.5

45

3.5   M Sodium bromide


0.1 M TRIS.HCl   pH 8.5

46

1.75   M Sodium bromide

 

0.1 M TRIS.HCl   pH 8.5

47

2.4   M Sodium malonate

 

0.1 M TRIS.HCl   pH 8.5

48

1.2   M Sodium malonate

 

0.1 M TRIS.HCl   pH 8.5


The Anions Suite

No

Precipitant

Salt

Buffer   

49

3.5 M Sodium nitrate


0.1   M Sodium acetate pH 4.6

50

1.75 M Sodium nitrate


0.1   M Sodium acetate pH 4.6

51

0.9 M Sodium phosphate

0.9 M Potassium phosphate

0.1   M Sodium acetate pH 4.6

52

0.45 M Sodium phosphate

0.45 M Potassium phosphate

0.1   M Sodium acetate pH 4.6

53

1.0 M Sodium succinate


0.1   M Sodium acetate pH 4.6

54

0.5 M Sodium succinate


0.1   M Sodium acetate pH 4.6

55

3.5 M Sodium nitrate


0.1   M MES pH 6.5

56

1.75 M Sodium nitrate


0.1   M MES pH 6.5

57

0.9 M Sodium phosphate

0.9 M Potassium phosphate

0.1   M MES pH 6.5

58

0.45 M Sodium phosphate

0.45 M Potassium phosphate

0.1   M MES pH 6.5

59

1.0 M Sodium succinate


0.1   M MES pH 6.5

60

0.5 M Sodium succinate


0.1   M MES pH 6.5

61

3.5 M Sodium nitrate


0.1   M HEPES pH 7.5

62

1.75 M Sodium nitrate


0.1   M HEPES pH 7.5

63

0.9 M Sodium phosphate

0.9 M Potassium phosphate

0.1   M HEPES pH 7.5

64

0.45 M Sodium phosphate

0.45 M Potassium phosphate

0.1   M HEPES pH 7.5

65

1.0 M Sodium succinate


0.1   M HEPES pH 7.5

66

0.5 M Sodium succinate


0.1   M HEPES pH 7.5

67

3.5 M Sodium nitrate


0.1   M TRIS.HCl pH 8.5

68

1.75 M Sodium nitrate


0.1   M TRIS.HCl pH 8.5

69

0.9 M Sodium phosphate

0.9 M Potassium phosphate

0.1   M TRIS.HCl pH 8.5

70

0.45 M Sodium phosphate

0.45 M Potassium phosphate

0.1   M TRIS.HCl pH 8.5

71

1.0 M Sodium succinate


0.1   M TRIS.HCl pH 8.5

72

0.5 M Sodium succinate


0.1   M TRIS.HCl pH 8.5

73

0.75 M Sodium sulfate


0.1   M Sodium acetate pH 4.6

74

0.37 M Sodium sulfate


0.1   M Sodium acetate pH 4.6

75

1.2 M K/Na tartrate


0.1   M Sodium tartrate pH 5.6

76

0.6 M K/Na tartrate


0.1   M Sodium tartrate pH 5.6

77

2.4 M Sodium thiocyanate


0.1   M Sodium acetate pH 4.6

78

1.2 M Sodium thiocyanate


0.1   M Sodium acetate pH 4.6

79

0.75 M Sodium sulfate


0.1   M MES pH 6.5

80

0.37 M Sodium sulfate


0.1   M MES pH 6.5

81

1.2 M K/Na tartrate


0.1   M MES pH 6.5

82

0.6 M K/Na tartrate


0.1   M MES pH 6.5

83

2.4 M Sodium thiocyanate


0.1   M MES pH 6.5

84

1.2 M Sodium thiocyanate


0.1   M MES pH 6.5

85

0.75 M Sodium sulfate


0.1   M HEPES pH 7.5

86

0.37 M Sodium sulfate


0.1   M HEPES pH 7.5

87

1.2 M K/Na tartrate


0.1   M HEPES pH 7.5

88

0.6 M K/Na tartrate


0.1   M HEPES pH 7.5

89

2.4 M Sodium thiocyanate


0.1   M HEPES pH 7.5

90

1.2 M Sodium thiocyanate


0.1   M HEPES pH 7.5

91

0.75 M Sodium sulfate


0.1   M TRIS.HCl pH 8.5

92

0.37 M Sodium sulfate


0.1   M TRIS.HCl pH 8.5

93

1.2 M K/Na tartrate


0.1   M TRIS.HCl pH 8.5

94

0.6 M K/Na tartrate


0.1   M TRIS.HCl pH 8.5

95

2.4 M Sodium thiocyanate


0.1   M TRIS.HCl pH 8.5

96

1.2 M Sodium thiocyanate


0.1   M TRIS.HCl pH 8.5

 

The Nucleix Suite

No

Precipitant

Salt 2

Salt 3

Buffer

1

2 M Lithium sulfate

    


0.05   M MES pH 5.6

2

2.5 M Ammonium sulfate

    


0.05   M MES pH 5.6

3

20%(v/v) MPD

    


0.05   M MES pH 5.6

4

10%(v/v) PEG 400

0.01 M Magnesium sulfate


0.05   M MES pH 5.6

5

5%(w/v) PEG 8000

0.01 M Magnesium chloride


0.05   M MES pH 5.6

6

20%(w/v) PEG 8000

0.01 M Magnesium chloride


0.05   M MES pH 5.6

7

15%(v/v) Isopropanol

    


0.05   M MES pH 6.0

8

0.6 M Sodium chloride

0.005 M Magnesium sulfate


0.05   M MES pH 6.0

9

10%(v/v) PEG 400

0.01 M Magnesium chloride


0.05   M MES pH 6.0

10

5%(w/v) PEG 4000

    


0.05   M MES pH 6.0

11

1 M Lithium sulfate

    


0.05   M Sodium cacodylate pH 6.0

12

1.8 M Lithium sulfate

    


0.05   M Sodium cacodylate pH 6.0

13

1.7 M Ammonium sulfate

    


0.05   M Sodium cacodylate pH 6.0

14

15%(v/v) Isopropanol

0.025 M Magnesium chloride


0.05   M Sodium cacodylate pH 6.0

15

5%(v/v) MPD

    


0.05   M Sodium cacodylate pH 6.0

16

30%(v/v) MPD

    


0.05   M Sodium cacodylate pH 6.0

17

10%(w/v) PEG 4000

0.01 M Calcium chloride


0.05   M Sodium cacodylate pH 6.0

18

1.3 M Lithium sulfate

    


0.05   M Sodium cacodylate pH 6.5

19

2 M Ammonium sulfate

    


0.05   M Sodium cacodylate pH 6.5

20

10%(v/v) Isopropanol

0.015 M Magnesium acetate


0.05   M Sodium cacodylate pH 6.5

21

10%(w/v) 1,6-Hexanediol

0.005 M Magnesium chloride


0.05   M Sodium cacodylate pH 6.5

22

15%(v/v) PEG 400

    


0.05   M Sodium cacodylate pH 6.5

23

10%(w/v) PEG 4000

0.01 M Magnesium chloride


0.05   M Sodium cacodylate pH 6.5

24

10%(w/v) PEG 4000

0.01 M Calcium chloride


0.05   M Sodium cacodylate pH 6.5

25

30%(w/v) PEG 4000

    


0.05   M Sodium cacodylate pH 6.5

26

10%(w/v) PEG 8000

0.1 M Magnesium acetate


0.05   M Sodium cacodylate pH 6.5

27

30%(w/v) PEG 8000

0.01 M Magnesium acetate


0.05   M Sodium cacodylate pH 6.5

28

1.6 M Lithium sulfate

    


0.05   M HEPES Sodium salt pH 7.0

29

4 M Lithium chloride

    


0.05   M HEPES Sodium salt pH 7.0

30

1.6 M Ammonium sulfate

    


0.05   M HEPES Sodium salt pH 7.0

31

25%(v/v) PEG MME 550

    


0.05   M HEPES Sodium salt pH 7.0

32

20%(w/v) 1,6-Hexanediol

0.01 M Magnesium chloride


0.05   M HEPES Sodium salt pH 7.0

33

30%(w/v) 1,6-Hexanediol

0.01 M Magnesium chloride


0.05   M HEPES Sodium salt pH 7.0

34

15%(v/v) MPD

0.005 M Magnesium sulfate


0.05   M HEPES Sodium salt pH 7.0

35

5%(v/v) PEG 400

0.01 M Magnesium chloride


0.05   M HEPES Sodium salt pH 7.0

36

10%(v/v) PEG 400

0.01 M Calcium chloride


0.05   M HEPES Sodium salt pH 7.0

37

20%(v/v) PEG 200

0.025 M Magnesium sulfate


0.05   M HEPES Sodium salt pH 7.0

38

5%(w/v) PEG 4000

0.15 M Magnesium acetate


0.05   M HEPES Sodium salt pH 7.0

39

5%(w/v) PEG 8000

0.02 M Magnesium chloride


0.05   M HEPES Sodium salt pH 7.0

40

1.6 M Ammonium sulfate

    


0.05   M TRIS.HCl pH 7.5

41

10%(v/v) PEG MME 550

0.015 M Magnesium chloride


0.05   M TRIS.HCl pH 7.5

42

5%(v/v) Isopropanol

    


0.05   M TRIS.HCl pH 7.5

43

10%(v/v) MPD

0.01 M Magnesium chloride


0.05   M TRIS.HCl pH 7.5

44

10%(w/v) PEG 4000

0.05 M Magnesium chloride


0.05   M TRIS.HCl pH 7.5

45

1.8 M Ammonium sulfate

    


0.05   M TRIS.HCl pH 8.5

46

35%(w/v) 1,6-Hexanediol

    


0.05   M TRIS.HCl pH 8.5

47

30%(v/v) PEG 400

0.01 M Magnesium chloride


0.05   M TRIS.HCl pH 8.5

48

30%(w/v) PEG 4000

0.01 M Calcium chloride

 

0.05   M TRIS.HCl pH 8.5

49

 

80 mM Magnesium chloride

    

0.05   M HEPES pH 7.5

 

The Nucleix Suite

50

9%(v/v)   Isopropanol

18   mM Magnesium chloride

1 mM Copper sulfate

0.05 M Sodium   cacodylate pH 6.0

51

9%(v/v)   Isopropanol

18   mM Magnesium chloride

1.8 mM    [Co(NH3)6]Cl3

0.05 M Sodium   cacodylate pH 6.5

52

9%(v/v)   Isopropanol

18   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

53

4.5%(v/v)   MPD

18   mM Magnesium chloride

0.9 mM    [Co(NH3)6]Cl3

0.05 M Sodium   cacodylate pH 7.0

54

5%(v/v)   PEG 400

36   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

55

10%(v/v)   Isopropanol

10   mM Magnesium chloride


0.05 M Sodium   succinate pH 5.5

56

15%(v/v)   Ethanol

20   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.0

57

15%(v/v)   Ethanol

20   mM Magnesium chloride

1.0 mM    [Co(NH3)6]Cl3

0.05 M Sodium   cacodylate pH 7.0

58

10%(v/v)   tert-Butanol

5   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 7.0

59

5%(v/v)   PEG 400

30   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 7.0

60

5%(v/v)   Isopropanol

100   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

61

20%(v/v)   Ethanol

10   mM Magnesium chloride


0.05 M TRIS pH   8.0

62

5%(w/v)   PEG 8000

20   mM Magnesium chloride


0.05 M HEPES pH   7.5

63

5%(w/v)   PEG 4000

20   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.0

64

10%(v/v)   Isopropanol

10   mM Magnesium chloride

5 mM Calcium chloride

0.05 M Sodium   cacodylate pH 6.0

65

5%(v/v)   PEG 400

9   mM Magnesium chloride

1.8 mM [Co(NH3)6]Cl3

0.05 M Sodium   cacodylate pH 7.0

0.9   mM Spermidine

66

10%(v/v)   Isopropanol

10   mM Magnesium chloride

1 mM Copper sulfate

0.05 M Sodium   cacodylate pH 6.5

67

10%(v/v)   1,5-Hexandiol

20   mM Magnesium chloride

2 mM Calcium chloride

0.05 M Sodium   cacodylate pH 6.0

68

10%(v/v)   Dioxane

15   mM Magnesium chloride


0.05 M HEPES pH   7.5

69

10%(v/v)   PEG 400

15   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.0

70

9%(v/v)   Isopropanol

2.5   mM Spermine


0.05 M Sodium   cacodylate pH 6.5

71


2.0   mM Spermine

80 mM Calcium chloride

0.05 M Sodium   cacodylate pH 6.5

72


5   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

73

1.3   M Lithium sulfate

30   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

74

5%(v/v)   Isopropanol



0.05 M Sodium   cacodylate pH 6.0

75

10%(v/v)   Ethanol

100   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

76

2.5   M Sodium chloride

10   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.0

77

5%(v/v)   Isopropanol

10   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

78

2.0   M Lithium sulfate

15   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

79

2.0   M Ammonium sulfate

20   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

80

3.0   M Ammonium sulfate

10   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

81

1.0   M Ammonium sulfate

15   mM Magnesium chloride


0.05 M HEPES pH   7.5

82

2.5   M Sodium chloride



0.05 M Sodium   cacodylate pH 6.0

83

2.0   M Lithium chloride

200   mM Calcium acetate


0.05 M Sodium   cacodylate pH 6.0

84

2.0   M Sodium chloride

15   mM Magnesium chloride

1.0 mM    [Co(NH3)6]Cl3

0.05 M Sodium   cacodylate pH 6.5

85

20%(w/v)   PEG 1000

200   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

86

1.0   M Sodium tartrate


    

0.05 M TRIS pH   7.5

87

2.5   M Sodium chloride


    

0.05 M TRIS pH   7.5

88

2.5   M Potassium chloride


    

0.05 M Sodium   cacodylate pH 6.0

89

15%(v/v)   Ethanol


    

0.05 M TRIS pH   8.0

90

2.0   M Lithium sulfate

15   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.0

91

25%(v/v)   MPD

20   mM Magnesium acetate

100 mM Sodium chloride

0.05 M Sodium   cacodylate pH 6.0

92

3.0   M Ammonium sulfate

20   mM Magnesium chloride


0.05 M Sodium   succinate pH 5.5

93

2.5   M Potassium chloride



0.05 M Sodium   cacodylate pH 6.5

94

1.5   M Lithium sulfate

50   mM Magnesium chloride


0.05 M Sodium   cacodylate pH 6.5

95

2.0   M Lithium chloride

1.0   mM Spermine

30 mM Calcium chloride

0.05 M Sodium   cacodylate pH 6.5

96


10   mM Magnesium chloride

    

0.05 M Sodium   cacodylate pH 6.5

The PACT Suit

Number

Precipitant

Salt

Buffer

 

1

25% w/v PEG 1500


0.1   M SPG buffer pH 4

 

2

25% w/v PEG 1500


0.1   M SPG buffer pH 5

 

3

25% w/v PEG 1500


0.1   M SPG buffer pH 6

 

4

25% w/v PEG 1500


0.1   M SPG buffer pH 7

 

5

25% w/v PEG 1500


0.1   M SPG buffer pH 8

 

6

25% w/v PEG 1500


0.1   M SPG buffer pH 9

 

7

20% w/v PEG 6000

0.2 M Sodium chloride

0.1   M Sodium acetate pH 5

 

8

20% w/v PEG 6000

0.2 M Ammonium chloride

0.1   M Sodium acetate pH 5

 

9

20% w/v PEG 6000

0.2 M Lithium chloride

0.1   M Sodium acetate pH 5

 

10

20% w/v PEG 6000

0.2 M Magnesium chloride

0.1   M Sodium acetate pH 5

 

11

20% w/v PEG 6000

0.2 M Calcium chloride

0.1   M Sodium acetate pH 5

 

12

20% w/v PEG 6000

0.01 M Zinc chloride

0.1   M Sodium acetate pH 5

 

13

25% w/v PEG 1500


0.1   M MIB buffer pH 4

 

14

25% w/v PEG 1500


0.1   M MIB buffer pH 5

 

15

25% w/v PEG 1500


0.1   M MIB buffer pH 6

 

16

25% w/v PEG 1500


0.1   M MIB buffer pH 7

 

17

25% w/v PEG 1500


0.1   M MIB buffer pH 8

 

18

25% w/v PEG 1500


0.1   M MIB buffer pH 9

 

19

20% w/v PEG 6000

0.2 M Sodium chloride

0.1   M MES pH 6

 

20

20% w/v PEG 6000

0.2 M Ammonium chloride

0.1   M MES pH 6

 

21

20% w/v PEG 6000

0.2 M Lithium chloride

0.1   M MES pH 6

 

22

20% w/v PEG 6000

0.2 M Magnesium chloride

0.1   M MES pH 6

 

23

20% w/v PEG 6000

0.2 M Calcium chloride

0.1   M MES pH 6

 

24

20% w/v PEG 6000

0.01 M Zinc chloride

0.1   M MES pH 6

 

25

25% w/v PEG 1500


0.1   M PCB buffer pH 4

 

26

25% w/v PEG 1500


0.1   M PCB buffer pH 5

 

27

25% w/v PEG 1500


0.1   M PCB buffer pH 6

 

28

25% w/v PEG 1500


0.1   M PCB buffer pH 7

 

29

25% w/v PEG 1500


0.1   M PCB buffer pH 8

 

30

25% w/v PEG 1500


0.1   M PCB buffer pH 9

 

31

20% w/v PEG 6000

0.2 M Sodium chloride

0.1   M HEPES pH 7

 

32

20% w/v PEG 6000

0.2 M Ammonium chloride

0.1   M HEPES pH 7

 

33

20% w/v PEG 6000

0.2 M Lithium chloride

0.1   M HEPES pH 7

 

34

20% w/v PEG 6000

0.2 M Magnesium chloride

0.1   M HEPES pH 7

 

35

20% w/v PEG 6000

0.2 M Calcium chloride

0.1   M HEPES pH 7

 

36

20% w/v PEG 6000

0.01 M Zinc chloride

0.1   M HEPES pH 7

 

37

25% w/v PEG 1500


0.1   M MMT buffer pH 4

 

38

25% w/v PEG 1500


0.1   M MMT buffer pH 5

 

39

25% w/v PEG 1500


0.1   M MMT buffer pH 6

 

40

25% w/v PEG 1500


0.1   M MMT buffer pH 7

 

41

25% w/v PEG 1500


0.1   M MMT buffer pH 8

 

42

25% w/v PEG 1500


0.1   M MMT buffer pH 9

 

43

20% w/v PEG 6000

0.2 M Sodium chloride

0.1   M Tris pH 8

 

44

20% w/v PEG 6000

0.2 M Ammonium chloride

0.1   M Tris pH 8

 

45

20% w/v PEG 6000

0.2 M Lithium chloride

0.1   M Tris pH 8

 

46

20% w/v PEG 6000

0.2 M Magnesium chloride

0.1   M Tris pH 8

 

47

20% w/v PEG 6000

0.2 M Calcium chloride

0.1   M Tris pH 8

 

48

20% w/v PEG 6000


0.1   M Tris pH 8

 

49

20% w/v PEG   3350

0.2 M Sodium   fluoride

 

 

50

20% w/v PEG 3350

0.2 M Sodium bromide


51

20% w/v PEG 3350

0.2 M Sodium iodide


52

20% w/v PEG 3350

0.2 M Potassium   thiocyanate


53

20% w/v PEG 3350

0.2 M Sodium nitrate


54

20% w/v PEG 3350

0.2 M Sodium formate


55

20% w/v PEG 3350

0.2 M Sodium acetate


56

20% w/v PEG 3350

0.2 M Sodium sulphate


57

20% w/v PEG 3350

0.2 M Potassium/sodium tartrate


58

20% w/v PEG 3350

0.2 M Sodium/potassium   phosphate


59

20% w/v PEG 3350

0.2 M Sodium citrate


60

20% w/v PEG 3350

0.2 M Sodium malonate


61

20% w/v PEG 3350

0.2 M Sodium fluoride

0.1 M Bis Tris propane pH 6.5

62

20% w/v PEG 3350

0.2 M Sodium bromide

0.1 M Bis Tris propane pH 6.5

63

20% w/v PEG 3350

0.2 M Sodium iodide

0.1 M Bis Tris propane pH 6.5

64

20% w/v PEG 3350

0.2 M Potassium   thiocyanate

0.1 M Bis Tris propane pH 6.5

65

20% w/v PEG 3350

0.2 M Sodium nitrate

0.1 M Bis Tris propane pH 6.5

66

20% w/v PEG 3350

0.2 M Sodium formate

0.1 M Bis Tris propane pH 6.5

67

20% w/v PEG 3350

0.2 M Sodium acetate

0.1 M Bis Tris propane pH 6.5

68

20% w/v PEG 3350

0.2 M Sodium sulphate

0.1 M Bis Tris propane pH 6.5

69

20% w/v PEG 3350

0.2 M Potassium/sodium   tartrate

0.1 M Bis Tris propane pH 6.5

70

20% w/v PEG 3350

0.2 M Sodium/potassium   phosphate

0.1 M Bis Tris propane pH 6.5

71

20% w/v PEG 3350

0.2 M Sodium citrate

0.1 M Bis Tris propane pH 6.5

72

20% w/v PEG 3350

0.2 M Sodium malonate

0.1 M Bis Tris propane pH 6.5

73

20% w/v PEG 3350

0.2 M Sodium fluoride

0.1 M Bis Tris propane pH 7.5

74

20% w/v PEG 3350

0.2 M Sodium bromide

0.1 M Bis Tris propane pH 7.5

75

20% w/v PEG 3350

0.2 M Sodium iodide

0.1 M Bis Tris propane pH 7.5

76

20% w/v PEG 3350

0.2 M Potassium   thiocyanate

0.1 M Bis Tris propane pH 7.5

77

20% w/v PEG 3350

0.2 M Sodium nitrate

0.1 M Bis Tris propane pH 7.5

78

20% w/v PEG 3350

0.2 M Sodium formate

0.1 M Bis Tris propane pH 7.5

79

20% w/v PEG 3350

0.2 M Sodium acetate

0.1 M Bis Tris propane pH 7.5

80

20% w/v PEG 3350

0.2 M Sodium sulphate

0.1 M Bis Tris propane pH 7.5

81

20% w/v PEG 3350

0.2 M Potassium/sodium   tartarte

0.1 M Bis Tris propane pH 7.5

82

20% w/v PEG 3350

0.2 M Sodium/potassium   phosphate

0.1 M Bis Tris propane pH 7.5

83

20% w/v PEG 3350

0.2 M Sodium citrate

0.1 M Bis Tris propane pH 7.5

84

20% w/v PEG 3350

0.2 M Sodium malonate

0.1 M Bis Tris propane pH 7.5

85

20% w/v PEG 3350

0.2 M Sodium fluoride

0.1 M Bis Tris propane pH 8.5

86

20% w/v PEG 3350

0.2 M Sodium bromide

0.1 M Bis Tris propane pH 8.5

87

20% w/v PEG 3350

0.2 M Sodium iodide

0.1 M Bis Tris propane pH 8.5

88

20% w/v PEG 3350

0.2 M Potassium   thiocyanate

0.1 M Bis Tris propane pH 8.5

89

20% w/v PEG 3350

0.2 M Sodium nitrate

0.1 M Bis Tris propane pH 8.5

90

20% w/v PEG 3350

0.2 M Sodium formate

0.1 M Bis Tris propane pH 8.5

91

20% w/v PEG 3350

0.2 M Sodium acetate

0.1 M Bis Tris propane pH 8.5

92

20% w/v PEG 3350

0.2 M Sodium sulphate

0.1 M Bis Tris propane pH 8.5

93

20% w/v PEG 3350

0.2 M Potassium/sodium   tartrate

0.1 M Bis Tris propane pH 8.5

94

20% w/v PEG 3350

0.2 M Sodium/potassium   phosphate

0.1 M Bis Tris propane pH 8.5

95

20% w/v PEG 3350

0.2 M Sodium citrate

0.1 M Bis Tris propane pH 8.5

96

20% w/v PEG 3350

0.2 M Sodium malonate

0.1 M Bis Tris propane pH 8.5









The Protein Complex Suite

No.

Precipitant 1

Precipitant 2

Salt

Buffer

1

25%(w/v)   PEG 350 MME



0.1 M TRIS pH 8

2

15%(v/v)   PEG 400


0.1   Calcium acetate

0.1 M MES pH 6

3

20%(v/v)   PEG 400


0.1   Lithium chloride

0.1 M HEPES pH   7.5

4

25%(v/v)   PEG 400



0.1 M TRIS pH 8

5

15%(w/v)   PEG 550 MME



0.1 M MES pH   6.5

6

25%(w/v)   PEG 1000


0.2   M Sodium chloride

0.1 M Na/K   phosphate pH 6.5

7

20%(w/v)   PEG 1500


0.1   M Ammonium sulfate

0.1 M TRIS pH   7.5

8

10%(w/v)   PEG 2000 MME


0.2   M Ammonium sulfate

0.1 M Sodium   acetate pH 5.5

9

20%(w/v)   PEG 2000 MME


0.2   M Sodium chloride

0.1 M MES pH 6

10

15%(w/v)   PEG 2000 MME


0.1   M Potassium chloride

0.1 M  TRIS pH 8

11

25%(w/v)   PEG 2000 MME



0.1 M HEPES pH   7.5

12

5%(w/v)   PEG 4000


0.2   M Sodium acetate

0.1 M Sodium   citrate pH 5.5

13

5%(w/v)   PEG 4000


0.2   M Lithium sulfate

0.1 M TRIS pH   7.5

14

10%(w/v)   PEG 4000


0.1   M Calcium acetate

0.1 M Sodium   acetate pH 4.5

15

10%(w/v)   PEG 4000


0.2   M Sodium acetate

0.1 M Sodium   citrate pH 5.5

16

10%(w/v)   PEG 4000


0.2   M Sodium chloride

0.1 M MES pH   6.5

17

10%(w/v)   PEG 4000


0.1   M Magnesium chloride

0.1 M HEPES pH   7.5

18

10%(w/v)   PEG 4000

10%   (v/v) Isopropanol

0.1 M HEPES pH   7

19

15%(w/v)   PEG 4000


0.2   M Ammonium acetate

0.1 M Sodium   acetate pH 4

20

15%(w/v)   PEG 4000


0.1   M Magnesium chloride

0.1 M Sodium   citrate pH 5

21

15%(w/v)   PEG 4000



0.1 M Sodium   cacodylate pH 6

22

15%(w/v)   PEG 4000


0.15   M Ammonium sulfate

0.1 M MES pH 6

23

15%(w/v)   PEG 4000



0.1 M HEPES pH   7

24

15%(w/v)   PEG 4000


0.1   M Magnesium chloride

0.1 M HEPES pH   7

25

15%(w/v)   PEG 4000


0.15   M Ammonium sulfate

0.1 M TRIS pH 8

26

20%(w/v)   PEG 4000



0.1 M Sodium   citrate pH 4.5

27

20%(w/v)   PEG 4000


0.2   M Ammonium acetate

0.1 M Sodium   acetate pH 5

28

20%(w/v)   PEG 4000


0.2   M Lithium sulfate

0.1 M MES pH 6

29

20%(w/v)   PEG 4000



0.1 M TRIS pH 8

30

20%(w/v)   PEG 4000


0.15   M Ammonium sulfate

0.1 M HEPES pH   7

31

20%(w/v)   PEG 4000

20%   (v/v) Isopropanol

0.1 M Sodium   citrate pH 5.6

32

20%(w/v)   PEG 4000


0.2   M Sodium chloride

0.1 M TRIS pH 8

33

25%(w/v)   PEG 4000



0.1 M Sodium   cacodylate pH 5.5

34

25%(w/v)   PEG 4000


0.15   M Ammonium sulfate

0.1 M MES pH   5.5

35

25%(w/v)   PEG 4000



0.1 M Sodium   cacodylate pH 6.5

36

25%(w/v)   PEG 4000


0.2   M Potassium iodide

0.1 M MES pH   6.5

37

25%(w/v)   PEG 4000


0.2   M Sodium chloride

0.1 M HEPES pH   7.5

38

10%(w/v)   PEG 5000 MME

12%   (v/v) 1-propanol

0.1 M MES pH   6.5

39

15%(w/v)   PEG 5000 MME


0.1   M Potassium chloride

0.1 M HEPES pH   7

40

20%(w/v)   PEG 5000 MME


0.2   M Ammonium sulfate

0.1 M TRIS pH   7.5

41

8%(w/v)   PEG 6000


0.1   M Magnesium chloride

0.1 M MES pH 6

42

8%(w/v)   PEG 6000


0.15   M Sodium chloride

0.1 M TRIS pH 8

43

15%(w/v)   PEG 6000



0.1 M Sodium   citrate pH 5.5

44

15%(w/v)   PEG 6000


0.1   M Magnesium acetate

0.1 M Sodium   cacodylate pH 6.5

45

15%(w/v)   PEG 6000

5%(v/v)   MPD

0.1 M MES pH   6.5

46

15%(w/v)   PEG 6000


0.1   M Potassium chloride

0.1 M HEPES pH   7.5

47

15%(w/v)   PEG 6000



0.1 M TRIS pH   7.5

48

20%(w/v)   PEG 6000



0.1 M TRIS pH   8.5

The Protein Complex Suite

Number

Precipitant 1

Precipitant 2

Salt

Buffer

49

8%(w/v)   PEG 8000


0.1   M Magnesium acetate

0.1 M Sodium   acetate pH 4.5

50

8%(w/v)   PEG 8000



0.1 M Sodium   citrate pH 5

51

8%(w/v)   PEG 8000


0.2   M Sodium chloride

0.1 M Sodium   cacodylate pH 6

52

8%(w/v)   PEG 8000



0.1 M HEPES pH   7

53

8%(w/v)   PEG 8000



0.1 M TRIS pH 8

54

12%(w/v)   PEG 8000


0.1   M Calcium acetate

0.1 M Sodium   cacodylate pH 5.5

55

12%(w/v)   PEG 8000

 

 

0.1 M NaH2PO4   pH 6.5

56

12%(w/v)   PEG 8000


0.1   M Magnesium acetate

0.1 M MOPS pH   7.5

57

12%(w/v)   PEG 8000


0.2   M Sodium chloride

0.1 M HEPES pH   7.5

58

12%(w/v)   PEG 8000


0.2   M Ammonium sulfate

0.1 M TRIS pH   8.5

59

20%(w/v)   PEG 8000



0.1 M Sodium   citrate pH 5

60

20%(w/v)   PEG 8000


0.2   M Ammonium sulfate

0.1 M MES pH   6.5

61

20%(w/v)   PEG 8000



0.1 M HEPES pH   7

62

20%(w/v)   PEG 8000


0.2   M Lithium chloride

0.1 M TRIS pH 8

63

10%(w/v)   PEG 10000


0.1   M Magnesium acetate

0.1 M MES pH   6.5

64

18%(w/v)   PEG 12000



0.1 M HEPES pH   7

65

8%(w/v)   PEG 20000


0.1   M Sodium chloride

0.1 M TRIS pH 8

66

15%(w/v)   PEG 20000



0.1 M HEPES pH   7

67

0.5   M Ammonium sulfate



0.1 M MES pH   6.5

68

1   M Ammonium sulfate



0.1 M Sodium   acetate pH 5

69

1   M Ammonium sulfate



0.1 M MES pH   6.5

70

1   M Ammonium sulfate



0.1 M TRIS pH 8

71

1.5   M Ammonium sulfate



0.1 M Sodium   acetate pH 5

72

1.5   M Ammonium sulfate



0.1 M HEPES pH   7

73

1.5   M Ammonium sulfate



0.1 M TRIS pH 8

74

2   M Ammonium sulfate



0.1 M Sodium   acetate pH 5

75

2   M Ammonium sulfate



0.1 M HEPES pH   7

76

2   M Ammonium sulfate



0.1 M TRIS pH 8

77

1   M Ammonium sulfate


1   M Potassium chloride

0.1 M HEPES pH   7

78

2   M Sodium formate



0.1 M Sodium   acetate pH 5

79

3   M Sodium formate



0.1 M TRIS pH   7.5

80

0.8   M Potassium

 

 

Sodium   phosphate pH 7.5

81

1.3   M Potassium

 

 

Sodium   phosphate pH 7.0

82

1.6   M Potassium

 

 

Sodium   phosphate pH 6.5

83

1   M Sodium acetate



0.1 M HEPES pH   7.5

84

1   M Sodium citrate



0.1 M HEPES pH   7

85

2   M Sodium chloride



0.1 M Sodium   citrate pH 6

86

1   M Lithium sulfate



0.1 M MES pH   6.5

87

1.6   M Lithium sulfate



0.1 M TRIS pH 8

88

1.4   M Sodium malonate



pH 6.0

89

1.2   M Na/K tartrate

 

 

0.1 M TRIS pH 8

90

1.6   M Magnesium sulfate



0.1 M MES pH   6.5

91

15%(v/v)   MPD

2%(w/v)   PEG 4000


0.1 M Sodium   acetate pH 5

92

25%(v/v)   MPD


0.05   M Calcium acetate

0.1 M Sodium   cacodylate pH 6

93

50%(v/v)   MPD



0.1 M Imidazole   pH 7

94

10%(v/v)   Isopropanol

5%(w/v)   PEG 4000

0.05   M  MgCl2

0.1 M MES pH   6.5

95

25%(v/v)   Isopropanol


0.2   M Ammonium acetate

0.1 M HEPES pH   7.5

96

15%(v/v)   Ethanol

5%(v/v)   MPD

0.1   M Sodium chloride

0.1 M TRIS pH 8

Natrix HT (HR2-131)

No

Salt

Buffer

Precipitant

1

0.01   M Magnesium chloride hexahydrate

0.05   M MES monohydrate pH 5.6

1.8 M Lithium   sulfate monohydrate

2

0.01   M Magnesium acetate tetrahydrate

0.05   M MES monohydrate pH 5.6

2.5 M (NH4)2SO4

3

0.1   M Magnesium acetate tetrahydrate

0.05   M MES monohydrate pH 5.6

20% v/v MPD

4

0.2   M KCl

0.01   M Magnesium sulfate heptahydrate

0.05   M MES monohydrate pH 5.6

10% v/v PEG 400

5

0.2   M KCl

0.01   M Magnesium chloride hexahydrate

0.05   M MES monohydrate pH 5.6

5% w/v PEG 8000

6

0.1   M (NH4)2SO4

0.01   M Magnesium chloride hexahydrate

0.05   M MES monohydrate pH 5.6

20% w/v PEG   8000

7

0.02   M Magnesium chloride hexahydrate

0.05   M MES monohydrate pH 6.0

15% v/v   2-Propanol

8

0.1   M NH4CH3CO2

0.005   M Magnesium sulfate heptahydrate

0.05   M MES monohydrate pH 6.0

0.6 M NaCl

9

0.1   M KCl

0.01   M Magnesium chloride hexahydrate

0.05   M MES monohydrate pH 6.0

10% v/v PEG 400

10

0.005   M Magnesium sulfate heptahydrate

0.05   M MES monohydrate pH 6.0

5% w/v PEG 4000

11

0.01   M Magnesium chloride hexahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

1.0 M Lithium   sulfate monohydrate

12

0.01   M Magnesium sulfate heptahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

1.8 M Lithium   sulfate monohydrate

13

0.015   M Magnesium acetate tetrahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

1.7 M (NH4)2SO4

14

0.1   M KCl

0.025   M Magnesium chloride hexahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

15% v/v   2-Propanol

15

0.04   M Magnesium chloride hexahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

5% v/v MPD

16

0.04   M Magnesium acetate tetrahydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

30% v/v MPD

17

0.2   M KCl

0.01   M Calcium chloride dihydrate

0.05   M Sodium cacodylate trihydrate pH 6.0

10% w/v PEG   4000

18

0.01   M Magnesium acetate tetrahydrate

0.05   M Sodium cacodylate trihydrate pH 6.5

1.3 M Lithium   sulfate monohydrate

19

0.01   M Magnesium sulfate heptahydrate

0.05   M Sodium cacodylate trihydrate pH 6.5

2.0 M (NH4)2SO4

20

0.1   M NH4CH3CO2

0.015   M Mg(CH3COO)2 • 4H2O

0.05   M Sodium cacodylate trihydrate pH 6.5

10% v/v   2-Propanol

21

0.2   M KCl

0.005   M Magnesium chloride hexahydrate

0.05   M Sodium cacodylate trihydrate pH 6.5

0.9 M 1,   6-Hexanediol

Natrix HT (HR2-131)

No

Salt

Buffer

Precipitant

22

0.08 M Magnesium acetate tetrahydrate

0.05 M Sodium cacodylate trihydrate pH   6.5

15%   v/v PEG 400

23

0.2 M KCl

0.01   M Magnesium chloride hexahydrate

0.05 M Sodium cacodylate trihydrate pH   6.5

10%   w/v PEG 4000

24

0.2 M NH4CH3CO2

0.01   M Calcium chloride dihydrate

0.05 M Sodium cacodylate trihydrate pH   6.5

10%   w/v PEG 4000

25

0.08 M Magnesium acetate tetrahydrate

0.05 M Sodium cacodylate trihydrate pH   6.5

30%   w/v PEG 4000

26

0.2 M KCl

0.1   M Magnesium acetate tetrahydrate

0.05 M Sodium cacodylate trihydrate pH   6.5

10%   w/v PEG 8000

27

0.2 M NH4CH3CO2

0.01   M Mg(CH3COO)2 • 4H2O

0.05 M Sodium cacodylate trihydrate pH   6.5

30%   w/v PEG 8000

28

0.05 M Magnesium sulfate hydrate

0.05 M HEPES Sodium pH 7.0

1.6   M Lithium sulfate monohydrate

29

0.01 M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

4.0   M Lithium chloride

30

0.01 M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

1.6   M (NH4)2SO4

31

0.005 M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

25%   v/v PEG monomethyl ether 550

32

0.2 M KCl

0.01   M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

1.7   M 1, 6-Hexanediol

33

0.2 M Ammonium chloride

0.01   M Magnesium chloride hexahydrate

0.05 M HEPES pH 7.0

2.5   M 1, 6-Hexanediol

34

0.1 M KCl

0.005   M Magnesium sulfate hydrate

0.05 M HEPES Sodium pH 7.0

15%   v/v MPD

35

0.1 M KCl

0.01   M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

5%   v/v PEG 400

36

0.1 M KCl

0.01   M Calcium chloride dihydrate

0.05 M HEPES Sodium pH 7.0

10%   v/v PEG 400

37

0.2 M KCl

0.025   M Magnesium sulfate hydrate

0.05 M HEPES Sodium pH 7.0

20%   v/v PEG 200

38

0.2 M Ammonium acetate

0.15   M Magnesium acetate tetrahydrate

0.05 M HEPES Sodium pH 7.0

5%   w/v PEG 4000

39

0.1 M Ammonium acetate

0.02   M Magnesium chloride hexahydrate

0.05 M HEPES Sodium pH 7.0

5%   w/v PEG 8000

40

0.01 M Magnesium chloride hexahydrate

0.05 M TRIS hydrochloride pH 7.5

1.6   M (NH4)2SO4

41

0.1 M KCl

0.015   M MgCl2·6H2O

0.05 M Tris-HCl pH 7.5

10%   v/v PEG monomethyl ether 550

42

0.01 M MgCl2·6H2O

0.05 M Tris-HCl pH 7.5

5%   v/v 2-Propanol

 


Natrix HT (HR2-131)

No

Salt

Buffer

Precipitant

Additive

43

0.05 M Ammonium acetate

0.01   M MgCl2·6H2O

0.05 M Tris-HCl pH 7.5

10% v/v MPD

 

44

0.2 M KCl

0.05   M MgCl2·6H2O

0.05 M Tris-HCl pH 7.5

10% w/v PEG 4000

 

45

0.025 M Magnesium sulfate hydrate

0.05 M Tris-HCl pH 8.5

1.8 M Ammonium sulfate

 

46

0.005 M Magnesium sulfate hydrate

0.05 M Tris-HCl pH 8.5

2.9 M 1, 6-Hexanediol

 

47

0.1 M KCl

0.01   M MgCl2·6H2O

0.05 M Tris-HCl pH 8.5

30% v/v PEG 400

 

48

0.2 M Ammonium chloride

0.01   M CaCl2·2H2O

0.05 M Tris-HCl pH 8.5

30% w/v PEG 4000

 

49

0.04 M Lithium chloride

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   5.5

30% v/v MPD

0.002   M Hexammine cobalt(III) chloride

50

0.08 M NaCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   5.5

35% v/v MPD

0.002   M Hexammine cobalt(III) chloride

51

0.012 M NaCl

0.08   M KCl

0.04 M Sodium cacodylate trihydrate pH   5.5

45% v/v MPD

0.002   M Hexammine cobalt(III) chloride

52

0.02 M MgCl2 6H2O

0.04 M Sodium cacodylate trihydrate pH   5.5

40% v/v MPD

0.002   M Hexammine cobalt(III) chloride

53

2 mM CaCl2·2H2O

0.05 M Sodium cacodylate trihydrate pH   6.0

1.8 M (NH4)2SO4

0.5   mM Spermine

54

 

0.05 M Sodium cacodylate trihydrate pH   6.0

35% v/v Tacsimate pH 6.0

0.001   M Spermine

55

0.1 M NaCl

0.05 M Sodium cacodylate trihydrate pH   6.0

10% w/v PEG 4000

0.0005   M Spermine

56

0.05 M KCl

0.05 M Sodium cacodylate trihydrate pH   6.0

10% w/v PEG 8000

0.0005 M Spermine,

0.0005 M   L-Argininamide dihydrochloride

57

0.1 M KCl

0.05 M Sodium cacodylate trihydrate pH   6.0

16% w/v PEG 1000

0.0005   M Spermine

58

5 mM MgCl2·6H2O

2 mM   CaCl2·2H2O

0.05 M Sodium cacodylate trihydrate pH   6.0

15% v/v 2-Propano

1   mM Spermine

59

0.075 M NaCl

2 mM   CaCl2·2H2O

0.05 M Sodium cacodylate trihydrate pH   6.0

30% w/v 1, 6-Hexanediol

0.   5 mM Spermine

60

0.02 M Magnesium sulfate hydrate

0.002   M Cobalt(II) chloride hexahydrate

0.05 M Sodium cacodylate trihydrate pH   6.0

25% v/v MPD

0.0005   M Spermine

 

 

Natrix HT (HR2-131)

No

Salt

Buffer

Precipitant

Additive

61

 

0.05 M Sodium cacodylate trihydrate pH   6.0

30% v/v MPD

 

62

0.08 M NaCl

0.012   M KCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   6.0

30% v/v MPD

0.012   M Spermine tetrahydrochloride

63

0.08 M NaCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   6.0

35% v/v MPD

0.012   M Spermine tetrahydrochloride

64

0.08 M Strontium chloride hexahydrate

0.04 M Sodium cacodylate trihydrate pH   6.0

35% v/v MPD

0.012   M Spermine tetrahydrochloride

65

0.08 M KCl

0.02   M Barium chloride dihydrate

0.04 M Sodium cacodylate trihydrate pH   6.0

40% v/v MPD

0.012   M Spermine tetrahydrochloride

66

0.08 M KCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   6.0

45% v/v MPD

0.012   M Spermine tetrahydrochloride

67

0.08 M NaCl

0.04 M Sodium cacodylate trihydrate pH   6.0

45% v/v MPD

0.012   M Spermine tetrahydrochloride

68

0.08 M NaCl

0.02   M Barium chloride dihydrate

0.04 M Sodium cacodylate trihydrate pH   6.0

45% v/v MPD

0.012   M Spermine tetrahydrochloride

69

0.012 M NaCl

0.08   M KCl

0.04 M Sodium cacodylate trihydrate pH   6.0

50% v/v MPD

0.012   M Spermine tetrahydrochloride

70

0.08 M KCl

0.04 M Sodium cacodylate trihydrate pH   6.0

55% v/v MPD

0.012   M Spermine tetrahydrochloride

71

0.018 M MgCl2·6H2O

0.05 M Sodium cacodylate trihydrate pH   6.5

10% v/v 2-Propanol

0.003   M Spermine

72

0.02 M MgCl2·6H2O

0.05 M MOPS pH 7.0

2.0 M Ammonium sulfate

0.0005   M Spermine

73

 

0.05 M HEPES sodium pH 7.0

40% v/v Tacsimate pH 7.0

2 mM Spermine

2 mM Hexammine   cobalt(III) chloride

74

0.02 M MgCl2·6H2O

0.05 M MOPS pH 7.0

55% v/v Tacsimate pH 7.0

0.002   M Hexammine cobalt(III) chloride

75

0.02 M MgCl2·6H2O

0.05 M Sodium cacodylate trihydrate pH   7.0

15% v/v 2-Propanol

0.001 M Hexammine cobalt(III)   chloride,

0.001 M   Spermine

76

0.005 M MgCl2·6H2O

0.05 M MOPS pH 7.0

25% v/v 1, 4-Dioxane

0.001   M Spermine

77

0.01 M MgCl2·6H2O,

0.002   M Barium chloride dihydrate

0.05 M MOPS pH 7.0

30% v/v 1, 4-Dioxane

 

78

1 mM MgCl2·6H2O

0.002   M CaCl2·2H2O

0.05 M MOPS pH 7.0

15% v/v MPD

 

79

0.08 M Strontium chloride hexahydrate

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   7.0

20% v/v MPD

0.012   M Spermine tetrahydrochloride

80

0.08 M NaCl

0.04 M Sodium cacodylate trihydrate pH   7.0

30% v/v MPD

0.012   M Spermine tetrahydrochloride

81

0.04 M Lithium chloride

0.08   M Strontium chloride hexahydrate

0.04 M Sodium cacodylate trihydrate pH   7.0

30% v/v MPD

0.012   M Spermine tetrahydrochloride

82

0.04 M Lithium chloride,

0.08   M Strontium chloride hexahydrate,

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   7.0

30% v/v MPD

0.012   M Spermine tetrahydrochloride

83

0.08 M NaCl

0.012   M KCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   7.0

35% v/v MPD

0.012   M Spermine tetrahydrochloride

84

0.012 M NaCl

0.08   M KCl

0.04 M Sodium cacodylate trihydrate pH   7.0

40% v/v MPD

0.012   M Spermine tetrahydrochloride

85

0.08 M NaCl

0.02   M Barium chloride dihydrate

0.04 M Sodium cacodylate trihydrate pH   7.0

40% v/v MPD

0.012   M Spermine tetrahydrochloride

86

0.08 M NaCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   7.0

40% v/v MPD

0.012   M Spermine tetrahydrochloride

87

0.08 M KCl,

0.02   M Barium chloride dihydrate

0.04 M Sodium cacodylate trihydrate pH   7.0

40% v/v MPD

0.012   M Spermine tetrahydrochloride

88

0.08 M KCl

0.02   M MgCl2·6H2O

0.04 M Sodium cacodylate trihydrate pH   7.0

50% v/v MPD

0.012   M Spermine tetrahydrochloride 

89

0.08 M KCl

0.04 M Sodium cacodylate trihydrate pH   7.0

60% v/v MPD

0.012   M Spermine tetrahydrochloride

90

0.02 M MgCl2·6H2O

0.002   M Cobalt(II) chloride hexahydrate

0.05 M HEPES sodium  pH 7.5

2.0 M (NH4)2SO4

1   mM Spermine

91

0.02 M MgCl2·6H2O

0.05 M PIPES pH 7.5

4% w/v PEG 8000

0.001   M Spermine  

92

0.015 M MgCl2·6H2O,

2 mM   Barium chloride dihydrate

0.05 M PIPES pH 7.5

7% v/v 2-Propanol

0.   5 mM Spermine

93

0.02 M MgCl2·6H2O

0.05 M PIPES pH 7.5

10% w/v 1, 6-Hexanediol

0.001   M Spermine

94

0.01 M MgCl2·6H2O

0.05 M HEPES sodium pH 7.5

15% v/v MPD

0.0015   M Spermine

95

0.2 M CaCl2·2H2O

0.05 M HEPES sodium pH 7.5

28% v/v PEG 400

0.002   M Spermine 

96

2 mM Copper(II) chloride dihydrate

0.05 M Tris-HCl pH 8.5

1.8 M Lithium sulfate monohydrate

0.5   mM Spermine  

 


SaltRx HT (HR2-136)

No

Salt

Buffer

1

1.8 M Sodium acetate trihydrate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

2

2.8 M Sodium acetate trihydrate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

3

1.5 M Ammonium chloride

0.1   M Sodium acetate trihydrate pH 4.6

4

1.5 M Ammonium chloride

0.1   M BIS-TRIS propane pH 7.0

5

1.5 M Ammonium chloride

0.1   M Tris pH 8.5

6

3.5 M Ammonium chloride

0.1   M Sodium acetate trihydrate pH 4.6

7

3.5 M Ammonium chloride

0.1   M BIS-TRIS propane pH 7.0

8

3.5 M Ammonium chloride

0.1   M Tris pH 8.5

9

2.2 M NaCl

0.1   M Sodium acetate trihydrate pH 4.6

10

2.2 M NaCl

0.1   M BIS-TRIS propane pH 7.0

11

2.2 M NaCl

0.1   M Tris pH 8.5

12

3.2 M NaCl

0.1   M Sodium acetate trihydrate pH 4.6

13

3.2 M NaCl

0.1   M BIS-TRIS propane pH 7.0

14

3.2 M NaCl

0.1   M Tris pH 8.5

15

1.0 M Ammonium citrate dibasic

0.1   M Sodium acetate trihydrate pH 4.6

16

1.8 M Ammonium citrate dibasic

0.1   M Sodium acetate trihydrate pH 4.6

17

1.0 M Ammonium citrate tribasic pH 7.0

0.1   M BIS-TRIS propane pH 7.0

18

2.0 M Ammonium citrate tribasic pH 7.0

0.1   M BIS-TRIS propane pH 7.0

19

0.7 M Sodium citrate tribasic   dihydrate

0.1   M BIS-TRIS propane pH 7.0

20

0.7 M Sodium citrate tribasic   dihydrate

0.1   M Tris pH 8.5

21

1.2 M Sodium citrate tribasic   dihydrate

0.1   M BIS-TRIS propane pH 7.0

22

1.2 M Sodium citrate tribasic   dihydrate

0.1   M Tris pH 8.5

23

0.4 M Magnesium formate dihydrate

0.1   M Sodium acetate trihydrate pH 4.6

24

0.4 M Magnesium formate dihydrate

0.1   M BIS-TRIS propane pH 7.0

25

0.4 M Magnesium formate dihydrate

0.1   M Tris pH 8.5

26

0.7 M Magnesium formate dihydrate

0.1   M BIS-TRIS propane pH 7.0

27

2.0 M Sodium formate

0.1   M Sodium acetate trihydrate pH 4.6

28

2.0 M Sodium formate

0.1   M BIS-TRIS propane pH 7.0

29

2.0 M Sodium formate

0.1   M Tris pH 8.5

30

3.5 M Sodium formate

0.1   M Sodium acetate trihydrate pH 4.6

31

3.5 M Sodium formate

0.1   M BIS-TRIS propane pH 7.0

32

3.5 M Sodium formate

0.1   M Tris pH 8.5

33

1.2 M DL-Malic acid pH 7.0

0.1   M BIS-TRIS propane pH 7.0

34

2.2 M DL-Malic acid pH 7.0

0.1   M BIS-TRIS propane pH 7.0

35

1.4 M Sodium malonate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

36

2.4 M Sodium malonate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

37

2.5 M Ammonium nitrate

0.1   M Sodium acetate trihydrate pH 4.6

38

2.5 M Ammonium nitrate

0.1   M BIS-TRIS propane pH 7.0

39

2.5 M Ammonium nitrate

0.1   M Tris pH 8.5

40

6.0 M Ammonium nitrate

0.1   M Sodium acetate trihydrate pH 4.6

41

6.0 M Ammonium nitrate

0.1   M BIS-TRIS propane pH 7.0

42

6.0 M Ammonium nitrate

0.1   M Tris pH 8.5

43

1.5 M Sodium nitrate

0.1   M Sodium acetate trihydrate pH 4.6

44

1.5 M Sodium nitrate

0.1   M BIS-TRIS propane pH 7.0

45

1.5 M Sodium nitrate

0.1   M Tris pH 8.5

46

4.0 M Sodium nitrate

0.1   M Sodium acetate trihydrate pH 4.6

47

4.0 M Sodium nitrate

0.1   M BIS-TRIS propane pH 7.0

48

4.0 M Sodium nitrate

0.1   M Tris pH 8.5

49

1.0 M Ammonium phosphate monobasic

0.1   M Sodium acetate trihydrate pH 4.6

50

1.8 M Ammonium phosphate monobasic

0.1   M Sodium acetate trihydrate pH 4.6

51

1.5 M Ammonium phosphate dibasic

0.1   M Tris pH 8.5

52

2.4 M Ammonium phosphate dibasic

0.1   M Tris pH 8.5

53

1.0 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 5.0

54

1.0 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 6.9

55

1.0 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 8.2

56

1.8 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 5.0

57

1.8 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 6.9

58

1.8 M Sodium phosphate monobasic   monohydrate

Potassium   phosphate dibasic / pH 8.2

59

0.5 M Succinic acid pH 7.0

0.1   M BIS-TRIS propane pH 7.0

60

1.0 M Succinic acid pH 7.0

0.1   M BIS-TRIS propane pH 7.0

61

1.5 M Ammonium sulfate

0.1   M Sodium acetate trihydrate pH 4.6

62

1.5 M Ammonium sulfate

0.1   M BIS-TRIS propane pH 7.0

63

1.5 M Ammonium sulfate

0.1   M Tris pH 8.5

64

2.5 M Ammonium sulfate

0.1   M Sodium acetate trihydrate pH 4.6

65

2.5 M Ammonium sulfate

0.1   M BIS-TRIS propane pH 7.0

66

2.5 M Ammonium sulfate

0.1   M Tris pH 8.5

67

0.8 M Lithium sulfate monohydrate

0.1   M Sodium acetate trihydrate pH 4.6

68

0.8 M Lithium sulfate monohydrate

0.1   M BIS-TRIS propane pH 7.0

69

0.8 M Lithium sulfate monohydrate

0.1   M Tris pH 8.5

70

1.5 M Lithium sulfate monohydrate

0.1   M Sodium acetate trihydrate pH 4.6

71

1.5 M Lithium sulfate monohydrate

0.1   M BIS-TRIS propane pH 7.0

72

1.5 M Lithium sulfate monohydrate

0.1   M Tris pH 8.5

73

1.0 M Magnesium sulfate hydrate

0.1   M Sodium acetate trihydrate pH 4.6

74

1.0 M Magnesium sulfate hydrate

0.1   M BIS-TRIS propane pH 7.0

75

1.0 M Magnesium sulfate hydrate

0.1   M Tris pH 8.5

76

1.8 M Magnesium sulfate hydrate

0.1   M Sodium acetate trihydrate pH 4.6

77

1.8 M Magnesium sulfate hydrate

0.1   M BIS-TRIS propane pH 7.0

78

1.8 M Magnesium sulfate hydrate

0.1   M Tris pH 8.5

79

0.7 M Ammonium tartrate dibasic

0.1   M Sodium acetate trihydrate pH 4.6

80

0.7 M Ammonium tartrate dibasic

0.1   M BIS-TRIS propane pH 7.0

81

0.7 M Ammonium tartrate dibasic

0.1   M Tris pH 8.5

82

1.0 M Ammonium tartrate dibasic

0.1   M Sodium acetate trihydrate pH 4.6

83

1.3 M Ammonium tartrate dibasic

0.1   M BIS-TRIS propane pH 7.0

84

1.4 M Ammonium tartrate dibasic

0.1   M Tris pH 8.5

85

0.6 M Potassium sodium tartrate   tetrahydrate

0.1   M BIS-TRIS propane pH 7.0

86

1.2 M Potassium sodium tartrate   tetrahydrate

0.1   M BIS-TRIS propane pH 7.0

87

0.6 M Potassium sodium tartrate   tetrahydrate

0.1   M Tris pH 8.5

88

1.2 M Potassium sodium tartrate   tetrahydrate

0.1   M Tris pH 8.5

89

0.5 M Potassium thiocyanate

0.1   M Sodium acetate trihydrate pH 4.6

90

0.5 M Potassium thiocyanate

0.1   M BIS-TRIS propane pH 7.0

91

0.5 M Potassium thiocyanate

0.1   M Tris pH 8.5

92

4.0 M Ammonium acetate

0.1   M Sodium acetate trihydrate pH 4.6

93

4.0 M Ammonium acetate

0.1   M BIS-TRIS propane pH 7.0

94

4.0 M Ammonium acetate

0.1   M Tris pH 8.5

95

35% v/v Tacsimate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

96

60% v/v Tacsimate pH 7.0

0.1   M BIS-TRIS propane pH 7.0

 


PEGRx HT (HR2-086)

No

Buffer

Polymer

1

0.1 M Citric acid pH 3.5

34%   v/v PEG 200

2

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

38%   v/v PEG 200

3

0.1 M HEPES pH 7.5

42%   v/v PEG 200

4

0.1 M Sodium acetate trihydrate pH 4.5

30%   v/v PEG 300

5

0.1 M BIS-TRIS pH 6.5

25%   v/v PEG 300

6

0.1 M Bicine pH 8.5

20%   v/v PEG 300

7

0.1 M Sodium acetate trihydrate pH 4.0

15%   v/v PEG 400

8

0.1 M MES monohydrate pH 6.0

22%   v/v PEG 400

9

0.1 M Tris pH 8.0

30%   v/v PEG 400

10

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

30%   v/v PEG MME 550

11

0.1 M Imidazole pH 7.0

25%   v/v PEG MME 550

12

0.1 M BIS-TRIS propane pH 9.0

20%   v/v PEG MME 550

13

0.1 M Sodium acetate trihydrate pH 4.0

10%   v/v Jeffamine® M-600® pH 7.0

14

0.1 M MES monohydrate pH 6.0

20%   v/v Jeffamine® M-600® pH 7.0

15

0.1 M Tris pH 8.0

30%   v/v Jeffamine® M-600® pH 7.0

16

0.1 M Citric acid pH 3.5

14%   w/v PEG 1000

17

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

22%   w/v PEG 1000

18

0.1 M HEPES pH 7.5

30%   w/v PEG 1000

19

0.1 M Sodium acetate trihydrate pH 4.5

30%   w/v PEG 1500

20

0.1 M BIS-TRIS pH 6.5

20%   w/v PEG 1500

21

0.1 M Bicine pH 8.5

15%   w/v PEG 1500

22

0.1 M Sodium acetate trihydrate pH 4.0

10%   w/v PEG MME 2000

23

0.1 M MES monohydrate pH 6.0

20%   w/v PEG MME 2000

24

0.1 M Tris pH 8.0

30%   w/v PEG MME 2000

25

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

30%   v/v Jeffamine® ED-2001 pH 7.0

26

0.1 M Imidazole pH 7.0

20%   v/v Jeffamine® ED-2001 pH 7.0

27

0.1 M BIS-TRIS propane pH 9.0

10%   v/v Jeffamine® ED-2001 pH 7.0

28

0.1 M Citric acid pH 3.5

25%   w/v PEG 3350

29

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

18%   w/v PEG 3350

30

0.1 M HEPES pH 7.5

12%   w/v PEG 3350

31

0.1 M Sodium acetate trihydrate pH 4.0

10%   w/v PEG 4000

32

0.1 M MES monohydrate pH 6.0

14%   w/v PEG 4000

33

0.1 M Tris pH 8.0

28%   w/v PEG 4000

34

0.1 M Sodium acetate trihydrate pH 4.5

30%   PEG MME 5000

35

0.1 M BIS-TRIS pH 6.5

20%   w/v PEG MME 5000

36

0.1 M Bicine pH 8.5

8%   w/v PEG MME 5000

37

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

10%   w/v PEG 6000

38

0.1 M Imidazole pH 7.0

20%   w/v PEG 6000

39

0.1 M BIS-TRIS propane pH 9.0

30%   w/v PEG 6000

40

0.1 M Citric acid pH 3.5

28%   w/v PEG 8000

41

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

16%   w/v PEG 8000

42

0.1 M HEPES pH 7.5

4%   w/v PEG 8000

43

0.1   M Sodium acetate trihydrate pH 4.5

10%   w/v PEG 10,000

44

0.1   M BIS-TRIS pH 6.5

16%   w/v PEG 10,000

45

0.1   M Bicine pH 8.5

20%   w/v PEG 10,000

46

0.1   M Sodium citrate tribasic dihydrate pH 5.0

18%   w/v PEG 20,000

47

0.1   M Imidazole pH 7.0

12%   w/v PEG 20,000

48

0.1   M BIS-TRIS propane pH 9.0,

8%   w/v PEG 20,000

 

No

Buffer

Buffer

Polymer

49

0.8 M Lithium sulfate monohydrate

0.1 M Sodium acetate trihydrate pH 4.0

4%   v/v PEG 200

50

0.2 M Lithium sulfate monohydrate

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

26%   v/v PEG 200

51

0.05 M CaCl2·2H2O

0.1 M MES monohydrate pH 6.0

45%   v/v PEG 200

52

28% v/v 2-Propanol

0.1 M BIS-TRIS pH 6.5

3%   v/v PEG 200

53

20% v/v Tacsimate pH 7.0

0.1 M HEPES pH 7.5

2%   v/v PEG 200

54

10% v/v 2-Propanol

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

26%   v/v PEG 400

55

0.2 M Ammonium acetate

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

24%   v/v PEG 400

56

0.2 M Ammonium sulfate

0.1 M BIS-TRIS pH 6.5

18%   v/v PEG 400

57

0.19 mM CYMAL®-7

0.1 M HEPES pH 7.5

40%   v/v PEG 400

58

6% v/v 2-Propanol

0.1 M Sodium acetate trihydrate pH 4.5

26%   v/v PEG MME 550

59

1.8 M Ammonium sulfate

0.1 M BIS-TRIS pH 6.5

2%   v/v PEG MME 550

60

0.15 M DL-Malic acid pH 7.0

0.1 M Imidazole pH 7.0

22%   v/v PEG MME 550

61

0.1 M Succinic acid pH 7.0

0.1 M Bicine pH 8.5

30%   v/v PEG MME 550

62

0.1 M Lithium sulfate monohydrate

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

20%   w/v PEG 1000

63

0.1 M Sodium malonate pH 8.0

0.1 M Tris pH 8.0

30%   w/v PEG 1000

64

4% v/v MPD

0.1 M Citric acid pH 3.5

20%   w/v PEG 1500

65

0.2 M L-Proline

0.1 M HEPES pH 7.5

24%   w/v PEG 1500

66

10% v/v 2-Propanol

0.1 M Bicine pH 8.5

30%   w/v PEG 1500

67

0.1 M NaCl

0.1 M BIS-TRIS propane pH 9.0

25%   w/v PEG 1500

68

0.02 M Nickel(II) chloride hexahydrate

0.02   M MgCl2·6H2O

0.02   M Cadmium chloride hydrate

0.1 M Sodium acetate trihydrate pH 4.5

24%   w/v PEG MME 2000

69

20% v/v 2-Propanol

0.1 M MES monohydrate pH 6.0

20%   w/v PEG MME 2000

70

0.2 M Ammonium citrate tribasic pH 7.0

0.1 M Imidazole pH 7.0

20%   w/v PEG MME 2000

71

4.0 M Potassium formate

0.1 M BIS-TRIS propane pH 9.0

2%   w/v PEG MME 2000

72

50% v/v Tacsimate pH 4.0

0.1 M Sodium acetate trihydrate pH 4.5

1%   w/v PEG 3350

73

0.10% w/v n-Octyl-β-D-glucoside

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

22%   w/v PEG 3350

74

2% v/v Tacsimate pH 7.0

5%   v/v 2-Propanol

0.1 M Imidazole pH 7.0

8%   w/v PEG 3350

75

2% v/v 1, 4-Dioxane

0.1 M Tris pH 8.0

15%   w/v PEG 3350

76

18% v/v 2-Propanol

0.1 M Sodium citrate tribasic   dihydrate pH 5.5

20%   w/v PEG 4000

77

6% v/v Tacsimate pH 6.0

0.1 M MES monohydrate pH 6.0

25%   w/v PEG 4000

78

0.2 M Magnesium formate dihydrate

0.1 M Sodium acetate trihydrate pH 4.0

18%   w/v PEG MME 5000

79

2% v/v PEG 400

0.1 M Imidazole pH 7.0

24%   w/v PEG MME 5000

80

0.2 M Sodium formate

0.1 M Bicine pH 8.5

20%   w/v PEG MME 5000

81

4% v/v 2-Propanol

0.1 M BIS-TRIS propane pH 9.0

20%   w/v PEG MME 5000

82

6% v/v Ethylene glycol

0.1 M Citric acid pH 3.5

10%   w/v PEG 6000

83

0.15 M Lithium sulfate monohydrate

0.1 M Citric acid pH 3.5

18%   PEG 6000

84

10% v/v 2-Propanol

0.1 M Sodium acetate trihydrate pH 4.0

22%   w/v PEG 6000

85

0.2 M NaCl

0.1 M Sodium acetate trihydrate pH 4.0

22%   w/v PEG 8000

86

20% v/v 2-Propanol

0.1 M Tris pH 8.0

5%   w/v PEG 8000

87

10% v/v PEG 200

0.1 M BIS-TRIS propane pH 9.0

18%   w/v PEG 8000

88

15% v/v 2-Propanol

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

10%   w/v PEG 10000

89

0.4 M Sodium malonate pH 6.0

0.1 M MES monohydrate pH 6.0

0.5%   w/v PEG 10000

90

0.2 M Potassium sodium tartrate   tetrahydrate

0.1 M BIS-TRIS pH 6.5

10%   w/v PEG 10000

91

5% v/v MPD

0.1 M HEPES pH 7.5

10%   w/v PEG 10000

92

0.2 M Ammonium acetate

0.1 M Tris pH 8.0

16%   w/v PEG 10000

93

5% v/v 2-Propanol

0.1 M Citric acid pH 3.5

6%   w/v PEG 20000

94

1.0 M Sodium malonate pH 5.0

0.1 M Sodium acetate trihydrate pH 4.5

2%   w/v PEG 20000

95

0.2 M MgCl2·6H2O

0.1 M Sodium citrate tribasic   dihydrate pH 5.0

10%   w/v PEG 20000

96

3% w/v Dextran sulfate sodium salt

0.1 M Bicine pH 8.5

15%   w/v PEG 20000

 


Crystal Screen HT (HR2-130)

No

Salt

Buffer

Precipitant

1

0.02 M CaCl2·2H2O

0.1 M Sodium acetate trihydrate pH 4.6

30%   v/v MPD

2

0.4 M Potassium sodium tartrate   tetrahydrate

 

 

3

0.4 M Ammonium phosphate monobasic

 

 

4

0.1 M Tris-HCl pH 8.5

2.0 M Ammonium sulfate

 

5

0.2 M Sodium citrate tribasic   dihydrate

0.1 M HEPES sodium pH 7.5

30%   v/v MPD

6

0.2 M MgCl2·6H2O

0.1 M Tris-HCl pH 8.5

30%   w/v PEG 4000

7

0.1 M Sodium cacodylate trihydrate pH   6.5

1.4 M Sodium acetate trihydrate

 

8

0.2 M Sodium citrate tribasic   dihydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

30%   v/v 2-Propanol

9

0.2 M Ammonium acetate

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

30%   w/v PEG 4000

10

0.2 M Ammonium acetate

0.1 M Sodium acetate trihydrate pH 4.6

30%   w/v PEG 4000

11

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

1.0 M Ammonium phosphate monobasic

 

12

0.2 M MgCl2·6H2O

0.1 M HEPES sodium pH 7.5

30%   v/v 2-Propanol

13

0.2 M Sodium citrate tribasic   dihydrate

0.1 M Tris-HCl pH 8.5

30%   v/v PEG 400

14

0.2 M CaCl2·2H2O

0.1 M HEPES sodium pH 7.5

28%   v/v PEG 400

15

0.2 M Ammonium sulfate

0.1 M Sodium cacodylate trihydrate pH   6.5

30%   w/v PEG 8000

16

0.1 M HEPES sodium pH 7.5

1.5 M Lithium sulfate monohydrate

 

17

0.2 M Lithium sulfate monohydrate

0.1 M Tris-HCl pH 8.5

30%   w/v PEG 4000

18

0.2 M Magnesium acetate tetrahydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

20%   w/v PEG 8000

19

0.2 M Ammonium acetate

0.1 M Tris-HCl pH 8.5

30%   v/v 2-Propanol

20

0.2 M Ammonium sulfate

0.1 M Sodium acetate trihydrate pH 4.6

25%   w/v PEG 4000

21

0.2 M Magnesium acetate tetrahydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

30%   v/v MPD

22

0.2 M Sodium acetate trihydrate

0.1 M Tris-HCl pH 8.5

30%   w/v PEG 4000

23

0.2 M MgCl2·6H2O

0.1 M HEPES sodium pH 7.5

30%   v/v PEG 400

24

0.2 M CaCl2·2H2O

0.1 M Sodium acetate trihydrate pH 4.6

20%   v/v 2-Propanol

25

0.1 M Imidazole pH 6.5

1.0 M Sodium acetate trihydrate

 

26

0.2 M Ammonium acetate

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

30%   v/v MPD

27

0.2 M Sodium citrate tribasic   dihydrate

0.1 M HEPES sodium pH 7.5

20%   v/v 2-Propanol

28

0.2 M Sodium acetate trihydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

30%   w/v PEG 8000

29

0.1 M HEPES sodium pH 7.5

0.8 M Potassium sodium tartrate   tetrahydrate

 

30

0.2 M Ammonium sulfate

30% w/v PEG 8000

 

31

0.2 M Ammonium sulfate

30% w/v PEG 4000

 

32

2.0 M Ammonium sulfate

 

 

33

4.0 M Sodium formate

 

 

34

0.1 M Sodium acetate trihydrate pH 4.6

2.0 M Sodium formate

 

35

0.1 M HEPES sodium pH 7.5

0.8 M Sodium phosphate monobasic   monohydrate

0.8   M Potassium phosphate monobasic

36

0.1 M Tris-HCl pH 8.5

8% w/v PEG 8000

 

37

0.1 M Sodium acetate trihydrate pH 4.6

8% w/v PEG 4000

 

38

0.1 M HEPES sodium pH 7.5

1.4 M Sodium citrate tribasic   dihydrate

 

 

 

 

 

39

0.1 M HEPES sodium pH 7.5

2% v/v PEG 400

2.0   M Ammonium sulfate

40

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

20% v/v 2-Propanol

20%   w/v PEG 4000

41

0.1 M HEPES sodium pH 7.5

10% v/v 2-Propanol

20%   w/v PEG 4000

42

0.05 M Potassium phosphate monobasic

20% w/v PEG 8000

 

43

30% w/v PEG 1500

 

 

44

0.2 M Magnesium formate dihydrate

 

 

45

0.2 M Zinc acetate dihydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

18%   w/v PEG 8000

46

0.2 M Calcium acetate hydrate

0.1 M Sodium cacodylate trihydrate pH   6.5

18%   w/v PEG 8000

47

0.1 M Sodium acetate trihydrate pH 4.6

2.0 M Ammonium sulfate

 

48

0.1 M Tris-HCl pH 8.5

2.0 M Ammonium phosphate monobasic

 

49

2.0 M NaCl

10% w/v PEG 6000

 

50

0.5 M NaCl

0.01 M MgCl2·6H2O

0.01   M Hexadecyltrimethylammonium bromide

51

25% v/v Ethylene glycol

 

 

52

35% v/v 1

4-Dioxane

 

53

2.0 M Ammonium sulfate

5% v/v 2-Propanol

 

54

1.0 M Imidazole pH 7.0

 

 

55

10% w/v PEG 1000

10% w/v PEG 8000

 

56

1.5 M NaCl

10% v/v Ethanol

 

57

0.1 M Sodium acetate trihydrate pH 4.6

2.0 M NaCl

 

58

0.2 M NaCl

0.1 M Sodium acetate trihydrate pH 4.6

30%   v/v MPD

59

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Sodium acetate trihydrate pH 4.6

1.0   M 1, 6-Hexanediol

60

0.1 M Cadmium chloride hydrate

0.1 M Sodium acetate trihydrate pH 4.6

30%   v/v PEG 400

61

0.2 M Ammonium sulfate

0.1 M Sodium acetate trihydrate pH 4.6

30%   w/v PEG MME 2000

62

0.2 M Potassium sodium tartrate   tetrahydrate

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

2.0   M (NH4)2SO4

63

0.5 M (NH4)2SO4

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

1.0   M Lithium sulfate monohydrate

64

0.5 M NaCl

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

2%   v/v Ethylene imine polymer

65

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

35% v/v tert-Butanol

 

66

0.01 M Iron(III) chloride hexahydrate

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

10%   v/v Jeffamine M-600

67

0.1 M Sodium citrate tribasic   dihydrate pH 5.6

2.5 M 1

6-Hexanediol

68

0.1 M MES monohydrate pH 6.5

1.6 M Magnesium sulfate heptahydrate

 

69

0.1 M Sodium phosphate monobasic   monohydrate,

0.1   M Potassium phosphate monobasic

0.1 M MES monohydrate pH 6.5

2.0   M NaCl

70

0.1 M MES monohydrate pH 6.5

12% w/v PEG 20000

 

71

1.6 M Ammonium sulfate

0.1 M MES monohydrate pH 6.5

10%   v/v 1, 4-Dioxane

72

0.05 M Cesium chloride

0.1 M MES monohydrate pH 6.5

30%   v/v Jeffamine M-600

73

0.01 M Cobalt(II) chloride hexahydrate

0.1 M MES monohydrate pH 6.5

1.8   M (NH4)2SO4

74

0.2 M Ammonium sulfate

0.1 M MES monohydrate pH 6.5

30%   w/v PEG MME 5000

75

0.1 M MES monohydrate pH 6.5

0.01 M Zinc sulfate heptahydrate

25%   v/v PEG MME 550

 

 

 

 

76

1.6 M Sodium citrate tribasic   dihydrate pH 6.5

 

 

77

0.5 M Ammonium sulfate

0.1 M HEPES pH 7.5

30%   v/v MPD

78

0.1 M HEPES pH 7.5

10% w/v PEG 6000

5%   v/v MPD

79

0.1 M HEPES pH 7.5

20% v/v Jeffamine M-600

 

80

0.1 M NaCl

0.1 M HEPES pH 7.5

1.6   M Ammonium sulfate

81

2.0 M Ammonium formate

0.1 M HEPES pH 7.5

 

82

0.05 M Cadmium sulfate hydrate

0.1 M HEPES pH 7.5

1.0   M Sodium acetate trihydrate

83

0.1 M HEPES pH 7.5

70% v/v MPD

 

84

0.1 M HEPES pH 7.5

4.3 M NaCl

 

85

0.1 M HEPES pH 7.5

8% v/v Ethylene glycol

10%   w/v PEG 8000

86

0.1 M HEPES pH 7.5

20% w/v PEG 10

000

87

0.2 M MgCl2·6H2O

0.1 M Tris pH 8.5

3.4   M 1, 6-Hexanediol

88

0.1 M Tris pH 8.5

25% v/v tert-Butanol

 

89

0.01 M Nickel(II) chloride hexahydrate

0.1 M Tris pH 8.5

1.0   M Lithium sulfate monohydrate

90

1.5 M Ammonium sulfate

0.1 M Tris pH 8.5

12%   v/v Glycerol

91

0.2 M Ammonium phosphate monobasic

0.1 M Tris pH 8.5

50%   v/v MPD

92

0.1 M Tris pH 8.5

20% v/v Ethanol

 

93

0.01 M Nickel(II) chloride hexahydrate

0.1 M Tris pH 8.5

20%   w/v PEG MME 2000

94

0.1 M NaCl

0.1 M Bicine pH 9.0

20%   v/v PEG MME 550

95

0.1 M Bicine pH 9.0

2.0 M MgCl2·6H2O

 

96

0.1 M Bicine pH 9.0

2% v/v 1, 4-Dioxane

10%   w/v PEG 20000

 


MBP-Fusion Protein Crystallization Screen

Salt/additive

Buffer

Precipitant

1

0.2 M MgCl2

MES 6.0

20% PEG6000

2

0.2 M CaCl2

MES 6.0

20% PEG6000

3

0.2 M Zn   acetate

MES 6.0

20% PEG6000

4

0.2 M Li   acetate

cacodylate   6.5

20% PEG6000

5

0.2 M Li   sulfate

MES 6.0

15% PEG6000

6

0.2 M Na   tartrate

MES 6.0

20% PEG6000

7

0.1 M Na   citrate

MES 6.0

30% PEG6000

8

0.2 M NH4   acetate

MES 6.0

20% PEG6000

9

0.2 M NH4   sulfate

Na acetate   4.6

15% PEG6000

10

0.2 M NH4   tartrate

MES 6.0

20% PEG6000

11

0.1 M Na/K   phosphate

MES 6.0

20% PEG6000

12

0.1 M NaCl,   10 mM MnCl2

cacodylate   6.5

20% PEG6000

13

0.2 M Na   acetate

CHES 9.5

20% PEG6000

14

0.2 M MgCl2

HEPES 7.4

20% PEG6000

15

0.2 M CaCl2

HEPES 7.4

20% PEG6000

16

0.2 M Zn   acetate

HEPES 6.5

20% PEG6000

17

0.2 M Li   acetate

HEPES 7.4

25% PEG6000

18

0.2 M Li   sulfate

HEPES 7.4

20% PEG6000

19

0.2 M Na   tartrate

HEPES 7.4

20% PEG6000

20

0.2 M Na   citrate

HEPES 7.4

15% PEG6000

21

0.2 M NH4   acetate

HEPES 7.4

20% PEG6000

22

0.2 M NH4   sulfate

cacodylate   6.5

20% PEG6000

23

0.2 M NH4   tartrate

HEPES 7.4

20% PEG6000

24

0.1 M Na/K   phosphate

HEPES 7.4

20% PEG6000

25

0.1 M Na   iodide

Na acetate   4.6

20% PEG8000

26

0.2 M Na   citrate

MES 6.0

20% PEG8000

27

0.2 M Na   malonate

cacodylate   6.5

20% PEG8000

28

0.2 M NH4   sulfate

MES 6.5

20% PEG8000

29

0.1 M Mg   formate

HEPES 7.0

20% PEG8000

30


HEPES 7.4

20% PEG8000

31

0.1 M K   nitrate

Tris 8.0

20% PEG8000

32

0.1 M NH4   formate

CHES pH 9.5

20% PEG8000

33

0.1 M Zn acetate

cacodylate   6.5

20% PEG8000

34

0.2 M NH4Cl

Na citrate   5.6

15% PEG8000

35

0.2 M NH4   acetate

Na acetate   5.0

15% PEG8000

36

0.2 M Na/K   tartrate

MES 5.0

15% PEG8000

37

0.1 M LiCl

Tris 8.5

15% PEG8000

38

0.1 M MgCl2

Tris 8.5

25% PEG4000

39

0.2 M NH4 NO3

Na acetate pH   4.6

25% PEG4000

40

0.1 M NaCl,   10% glycerol

cacodylate   6.5

25% PEG4000

41

0.2 M Na   malonate

Tris 8.0

25% PEG4000

42

0.2 M Li   sulfate

MES 6.0

20% PEG4000

43

0.2 M Na   tartrate

HEPES 7.0

20% PEG4000

44

0.2 M Na   formate

HEPES 7.4

20% PEG4000

45

5%   isopropynol

Tris 8.5

15% PEG4000

46

0.1 M Zn   acetate

cacodylate   7.0

15% PEG4000

47

0.2 Li   acetate

Na citrate   5.6

15% PEG4000

48


HEPES 7.4

20% PEG10000

49


cacodylate   6.5

15% PEG10000

50


Na citrate   5.6

10% PEG10000

51

0.2 M NH4   citrate

HEPES 7.0

10% PEG10000

52

0.2 M K   acetate

Tris 8.0

10% PEG10000

53

10% glycerol

Na citrate   5.6

2.5 M NH4 sulfate

54


MES 6.0

2.5 M NH4 sulfate

55


HEPES 7.0

2.5 M NH4 sulfate

56


Tris 8.0

2.5 M NH4 sulfate

57


Na acetate   4.6

2.0 M NH4 sulfate

58


MES 5.5

2.0 M NH4 sulfate

59


cacodylate   6.5

2.0 M NH4 sulfate

60


HEPES 7.4

2.0 M NH4 sulfate

61


Tris 8.5

2.0 M NH4 sulfate

62

0.2 M MgCl2

Na citrate   5.6

1.5 M NH4 sulfate

63

10 mM Zn   acetate

MES 6.0

1.5 M NH4 sulfate

64

10 mM MnCl2

HEPES 7.0

1.5 M NH4 sulfate

65

10 mM CaCl2

Tris 8.0

1.5 M NH4 sulfate

66



1.8 M NaCl, 30% sucrose

67

50 mM Cd Cl2

HEPES 7.4

1.0 M Na acetate

68

0.2 M NH4   sulfate

MES 6.5

1.0 M Na acetate

69

0.2 M Na   acetate, 10 mM MgCl2

MES 6.0

20% PEG5000 MME

70

 0.2 M NH4 tartrate

phosphate 7.9

20% PEG2000 MME

71

0.2 KCl

Na citrate   5.6

20% PEG2000 MME

72

0.2 M NH4   sulfate

Na acetate   4.6

20% PEG2000 MME

73

0.2 M NH4   nitrate

cacodylate   6.5

20% PEG2000 MME

74

0.1 M NaCl

Na acetate   4.6

20% PEG2000 MME

75

0.1 M NaF

Tris 8.5

20% PEG2000 MME

76

0.1 M Na/K   phosphate

HEPES 7.0

20% PEG1000

77

0.2 M Na   malonate

MES 6.0

20% PEG1000

78

0.2 M NH4   sulfate

Na citrate   5.6

30% PEG550 MME

79

0.2 M NH4   sulfate

Na acetate   4.6

30% PEG550 MME

80

0.1 M MgCl2

cacodylate   6.5

30% PEG550 MME

81

0.1 M KCl

Na acetate   4.6

30% PEG550 MME

82


Tris 8.5

30% PEG550 MME

83

10 mM CaCl2

HEPES 7.4

35% PEG550 MME

84

10 mM Zn   acetate, 0.1 M NaCl

Na citrate   5.6

30% PEG400

85

0.1 M MgCl2

cacodylate   6.5

30% PEG400

86

0.2 M   ammonium sulfate

HEPES 7.0

25% PEG400, 25% glycerol

87


Na citrate   5.6

20% PEG400, 20% sucrose

88

2% MPD, 50 mM   MnCl2

MES 6.5

40% PEG400

89

50 mM MgCl2

HEPES 7.4

40% PEG400

90

50 mM CaCl2

Na acetate   4.6

40% PEG400

91

0.1 M CaCl2

HEPES 7.0

30% MPD

92

0.1 M NaCl

Na citrate   5.6

30% MPD

93

0.2 M NH4   sulfate

cacodylate   6.5

25% MPD

94

0.1 M K   nitrate

MES 5.5

20% MPD

95

0.1 M Na/K   phosphate

Na acetate   4.6

20% MPD

96

10 mM MgCl2,   0.5% Jeffamine

cacodylate   6.0

1M Li sulfate

No.

salts

Buffer

Precipitant

1

0.2 M Sodium   fluoride


 20% w/v PEG 3350

2

0.2 M   Potassium fluoride


 20% w/v PEG 3350

3

0.2 M   Ammonium fluoride


 20% w/v PEG 3350

4

0.2 M Lithium   chloride


 20% w/v PEG 3350

5

0.2 M   Magnesium chloride hexahydrate


 20% w/v PEG 3350

6

0.2 M Sodium   chloride


 20% w/v PEG 3350

7

0.2 M Calcium   chloride dihydrate


 20% w/v PEG 3350

8

0.2 M   Potassium chloride


 20% w/v PEG 3350

9

0.2 M   Ammonium chloride


 20% w/v PEG 3350

10

0.2 M Sodium   iodide


 20% w/v PEG 3350

11

0.2 M   Potassium iodide


 20% w/v PEG 3350

12

0.2 M   Ammonium iodide


 20% w/v PEG 3350

13

0.2 M Sodium   thiocyanate


 20% w/v PEG 3350

14

0.2 M   Potassium thiocyanate


 20% w/v PEG 3350

15

0.2 M Lithium   nitrate


 20% w/v PEG 3350

16

0.2 M   Magnesium nitrate hexahydrate


 20% w/v PEG 3350

17

0.2 M Sodium   nitrate


 20% w/v PEG 3350

18

0.2 M   Potassium nitrate


 20% w/v PEG 3350

19

0.2 M   Ammonium nitrate


 20% w/v PEG 3350

20

0.2 M   Magnesium formate dihydrate


 20% w/v PEG 3350

21

0.2 M Sodium   formate


 20% w/v PEG 3350

22

0.2 M   Potassium formate


 20% w/v PEG 3350

23

0.2 M   Ammonium formate


 20% w/v PEG 3350

24

0.2 M Lithium   acetate dihydrate


 20% w/v PEG 3350

25

0.2 M   Magnesium acetate tetrahydrate


 20% w/v PEG 3350

26

0.2 M Zinc   acetate dihydrate


 20% w/v PEG 3350

27

0.2 M Sodium acetate   trihydrate


 20% w/v PEG 3350

28

0.2 M Calcium   acetate hydrate


 20% w/v PEG 3350

29

0.2 M   Potassium acetate


 20% w/v PEG 3350

30

0.2 M   Ammonium acetate


 20% w/v PEG 3350

31

0.2 M Lithium   sulfate monohydrate


 20% w/v PEG 3350

32

0.2 M Magnesium   sulfate heptahydrate


 20% w/v PEG 3350

33

0.2 M Sodium   sulfate decahydrate


 20% w/v PEG 3350

34

0.2 M   Potassium sulfate


 20% w/v PEG 3350

35

0.2 M   Ammonium sulfate


 20% w/v PEG 3350

36

0.2 M Sodium   tartrate dibasic dihydrate


 20% w/v PEG 3350

37

0.2 M   Potassium sodium tartrate


 20% w/v PEG 3350

38

0.2 M   Ammonium tartrate dibasic


 20% w/v PEG 3350

39

0.2 M Sodium   dihydrogen phosphate monohydrate


 20% w/v PEG 3350

40

0.2 M Sodium   phosphate dibasic dihydrate


 20% w/v PEG 3350

41

0.2 M Potassium   phosphate monobasic


 20% w/v PEG 3350

 


PEG/Ion Screen (HR2-126 + HR2-098)

42

0.2 M   Potassium phosphate dibasic

 

 20% w/v PEG 3350

43

0.2 M   Ammonium phosphate monobasic

 

 20% w/v PEG 3350

44

0.2 M   Ammonium phosphate dibasic

 

 20% w/v PEG 3350

45

0.2 M Lithium   citrate tribasic tetrahydrate

 

 20% w/v PEG 3350

46

0.2 M Sodium   citrate tribasic dihydrate

 

 20% w/v PEG 3350

47

0.2 M   Potassium citrate tribasic

 

 20% w/v PEG 3350

48

0.2 M   Ammonium citrate dibasic

 

 20% w/v PEG 3350

49

0.1 M Sodium   malonate pH 4.0


 12% w/v PEG 3350

50

0.2 M Sodium   malonate pH 4.0


 20% w/v PEG 3350

51

0.1 M Sodium   malonate pH 5.0


 12% w/v PEG 3350

52

0.2 M Sodium   malonate pH 5.0


 20% w/v PEG 3350

53

0.1 M Sodium   malonate pH 6.0


 12% w/v PEG 3350

54

0.2 M Sodium   malonate pH 6.0


 20% w/v PEG 3350

55

0.1 M Sodium   malonate pH 7.0


 12% w/v PEG 3350

56

0.2 M Sodium   malonate pH 7.0


 20% w/v PEG 3350

57

4% v/v   Tacsimate pH 4.0


 12% w/v PEG 3350

58

8% v/v   Tacsimate pH 4.0


 20% w/v PEG 3350

59

4% v/v   Tacsimate pH 5.0


 12% w/v PEG 3350

60

8% v/v   Tacsimate pH 5.0


 20% w/v PEG 3350

61

4% v/v   Tacsimate pH 6.0


 12% w/v PEG 3350

62

8% v/v   Tacsimate pH 6.0


 20% w/v PEG 3350

63

4% v/v   Tacsimate pH 7.0


 12% w/v PEG 3350

64

8% v/v   Tacsimate pH 7.0


 20% w/v PEG 3350

65

4% v/v   Tacsimate pH 8.0


 12% w/v PEG 3350

66

8% v/v   Tacsimate pH 8.0


 20% w/v PEG 3350

67

0.1 M   Succinic acid pH 7.0


 12% w/v PEG 3350

68

0.2 M   Succinic acid pH 7.0


 20% w/v PEG 3350

69

0.1 M   Ammonium citrate tribasic pH 7


 12% w/v PEG 3350

70

0.2 M   Ammonium citrate tribasic pH 7


 20% w/v PEG 3350

71

0.1 M   DL-Malic acid pH 7.0


 12% w/v PEG 3350

72

0.2 M   DL-Malic acid pH 7.0


 20% w/v PEG 3350

73

0.1 M Sodium   acetate trihydrate pH 7


 12% w/v PEG 3350

74

0.2 M Sodium   acetate trihydrate pH 7


 20% w/v PEG 3350

75

0.1 M Sodium   formate pH 7.0


 12% w/v PEG 3350

76

0.2 M Sodium   formate pH 7.0


 20% w/v PEG 3350

77

0.1 M   Ammonium tartrate dibasic pH 7


 12% w/v PEG 3350

78

0.2 M   Ammonium tartrate dibasic pH 7


 20% w/v PEG 3350

79

2% v/v   Tacsimate pH 4.0

 0.1 M Sodium acetate trihydrate pH 4.6

 16% w/v PEG 3350

80

2% v/v   Tacsimate pH 5.0

 0.1 M Sodium citrate tribasic dihydrate pH   5.6

 16% w/v PEG 3350

81

2% v/v   Tacsimate pH 6.0

 0.1 M BIS-TRIS pH 6.5

 20% w/v PEG 3350

 


PEG/Ion Screen (HR2-126 + HR2-098)

82

2% v/v   Tacsimate pH 7.0

 0.1 M HEPES pH 7.5

 20% w/v PEG 3350

83

2% v/v   Tacsimate pH 8.0

 0.1 M Tris pH 8.5

 16% w/v PEG 3350

84

(0.07 M   Citric acid

 0.03 M BIS-TRIS propane)/pH 3.4

 16% w/v PEG 3350

85

(0.06 M   Citric acid

 0.04 M BIS-TRIS propane)/pH 4.1

 16% w/v PEG 3350

86

(0.05 M   Citric acid

 0.05 M BIS-TRIS propane)/pH 5.0

 16% w/v PEG 3350

87

(0.04 M   Citric acid

 0.06 M BIS-TRIS propane)/pH 6.4

 20% w/v PEG 3350

88

(0.03 M   Citric acid

 0.07 M BIS-TRIS propane)/pH 7.6

 20% w/v PEG 3350

89

(0.02 M   Citric acid

 0.08 M BIS-TRIS propane)/pH 8.8

 16% w/v PEG 3350

90

0.02 M   Calcium chloride dihydrate,

0.02 M   Cadmium chloride hydrate,

0.02 M Cobalt   (II) chloride hexahydrate


 20% w/v PEG 3350

91

0.01 M   Magnesium chloride hexahydrate,

0.005 M   Nickel (II) chloride hexahydrate

0.1 M HEPES   sodium pH 7.0

 15% w/v PEG 3350

92

0.02 M Zinc   chloride


 20% w/v PEG 3350

93

0.15 M Cesium   chloride


 15% w/v PEG 3350

94

0.2 M Sodium   bromide


 20% w/v PEG 3350

95

1% w/v   Tryptone, 0.001 M Sodium azide

0.05 M HEPES   sodium pH 7.0

 12% w/v PEG 3350

96

1% w/v   Tryptone, 0.001 M Sodium azide

0.05 M HEPES   sodium pH 7.0

 20% w/v PEG 3350